洛汀新对阿霉素肾病大鼠肾血管紧张素Ⅱ-Ⅰ型受体基因表达的影响  

THE EFFECT OF LOTENSIN ON THE EXPRESSION OF ANGIOTENSIN Ⅱ TYPE Ⅰ RECEPTOR GENE IN RATS WITH ADRIAMYCIN-INDUCED NEPHROSIS

作　　者：刘娅[1,2] 尹忠诚 卢义侠 丁洁[1,2] 

机构地区：[1]徐州市第四人民医院儿科 [2]北京医科大学第一医院儿科

出　　处：《徐州医学院学报》1998年第6期451-453,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金

摘　　要：目的观察血管紧张素转化酶抑制剂洛汀新对阿霉素肾病（ＡＮ）大鼠肾脏血管紧张素Ⅱ（ＡＴⅡ）浓度及受体表达的影响。方法采用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术检测大鼠肾组织血管紧张素Ⅱ－Ⅰ型受体（ＡＴ１Ｒ）ｍＲＮＡ表达情况，以放射免疫分析法测定血浆及肾组织ＡＴⅡ含量。结果洛汀新可降低ＡＮ大鼠血浆及肾组织ＡＴⅡ含量，使肾脏ＡＴ１ＲｍＲＮＡ表达增强。结论洛汀新对ＡＮ大鼠肾脏ＡＴ１ＲｍＲＮＡ表达的影响与降低血浆及肾组织ＡＴⅡ含量有关。Objective To exemina the effect of LOTENSIN (an angiotensin converting enzyme inhibitor,ACEI) on angiotensin Ⅱ (AT Ⅱ) and its receptor in rats with adriamycin-induced nephrosis (AN).Methods The expression of AT Ⅱ type 1 receptor mRNA (AT 1R mRNA) in renal tissue was measured by RT-PCR.The concentration of AT Ⅱ in plasma and renal tissue was determined by RIA.Results Lotensin decreased the content of AT Ⅱ both in plasma and in kidney,and increased the expression of AT 1R mRNA in kidney.Conclusion The lowering of AT Ⅱ content in plasma and renal tissue is related to the effect of lotensin on the expression of AT 1R mRNA in the kidneys of AN rats.

关 键 词：洛汀新 阿霉素肾病 ATⅡ AT1R 肾疾病 MRNA 

分 类 号：R692[医药卫生—泌尿科学]