参三七皂甙Rb1、Rg1预适应对肥厚心肌细胞缺氧复氧损伤细胞凋亡的影响  

Effect of Panax notoginsenoside Rbl and Rgl on cell apoptosis of hypoxia - reoxygenafion injury in angiotensin H induced hypertrophied neonatal rat myocytes

作　　者：包金风[1] 张晓文[2] 卢新政[2] 饶曼人[2] 

机构地区：[1]徐州医学院药理教研室,江苏徐州221004 [2]南京医科大学药理教研室,江苏南京210029

出　　处：《甘肃医药》2010年第2期121-124,共4页Gansu Medical Journal

摘　　要：目的：研究参三七皂苷Rb1、Rg1药理性预适应对乳鼠肥厚心肌细胞缺氧／复氧损伤（A／R）细胞凋亡的影响。方法：采用血管紧张素Ⅱ诱导的肥厚心肌细胞A／R模型，心肌细胞随机分组：空白对照组，A／R模型对照组（组），参三七皂苷Rb1、Rg10．01—10μmol／L药物预适应组（预适应48h后进行A／R处理）；用流式细胞术观察心肌细胞凋亡率，电镜观察细胞超微结构。结果：与A／R对照组相比参三七皂苷Rb1、Rg10．0110μmol／L预适应48h后，细胞凋亡率呈浓度依赖性下降，Rb1、Rg1最大凋亡抑制率分别为86．68％和85．26％（P〈0．01）。电镜超微结构观察显示参三七皂苷Rb1、Rg1组细胞凋亡明显减轻，细胞结构完整，细胞基本结构无损害。结论：参三七皂苷Rb1、Rg1药理性预适应具有明显抑制细胞凋亡、改善心肌细胞缺氧／复氧损伤作用。Objective： To investigate the effect of Panax notoginsenoside Rb1 and Rg1 pharmacologic preconditioning on apeptosis in hypoxia/reoxygenation（A/R） injury in angiotensin Ⅱ induced hypertrophic myocardial cells of neonatal rat. Methods： Myocardial cells were divided into control group, A/R model group, Panax notoginsenoside RbI and Rg1 drug preconditioning groups （with Rb1 and Rgl0. 01 - 10μmol/L before A/R 48h ）. Apoptotic rate of myocardial cells were observed with flow cytometry, ultrastructure of myocardial cells were observed with electron microscope. Results： Apoptotic rate of myocardial cells of 0.01 - 10μmol/L Panax notoginsenoside Rb1 and Rg1 concentration - dependent decreased after 48h of preconditioning in which the maximum apoptoSe rate was 86.68 % and 85.26 % respectively （ P 〈 0. 01）, cell basic structure of panax notoginsenoside Rbt and RgI groups integrated in ultrastructure with electron microscope. Conclusion： Panax notoginsenoside Rb1 and Rg1 pharmacologic preconditioning can obviously inhibit apeptosis in angiotensin Ⅱ induced hypertrophied neonatal rat myocytes, which improved protective effect for myocardial hypoxia/reoxygenation injury.

关 键 词：参三七皂苷Rb1、Rg1 缺氧/复氧损伤 细胞凋亡 预适应 

分 类 号：R511.7[医药卫生—内科学]