大冶铜绿山鸭跖草居群的遗传变异初步分析  

Genetic variation of commelina communis L. populations in Tonglushan,Daye City

作　　者：陈国庆[1] 姚发兴[1] 冯坤[1] 江威[1] 

机构地区：[1]湖北师范学院生命科学学院,湖北黄石435002

出　　处：《湖北师范学院学报（自然科学版）》2010年第1期5-9,共5页Journal of Hubei Normal University(Natural Science)

基　　金：国家自然科学青年基金项目(30900157);湖北省教育厅优秀中青年人才项目(Q20082201);湖北师范学院人才引进项目(2007F13);优秀中青年科技创新团队项目

摘　　要：采用SRAP分子标记,对来自大冶铜绿山古铜矿冶炼渣堆上的鸭跖草居群(YL)进行遗传多样性检测,并以来自附近农田的居群(NT)作为对照。选取2对SRAP引物,共检测到24个清晰稳定的位点,其中20个为多态位点。进一步分析表明,YL居群的多样性水平(H=0.161,I=0.231)明显高于NT居群(H=0.094,I=0.145)。UPGMA分析发现所有鸭跖草个体均按其居群来源聚类在一起,PcoA分析支持UPGMA聚类结果,表明YL居群和NT居群在遗传变异基础上存在明显差异,推测是由于定居在古冶炼渣堆上的鸭跖草居群在长期进化过程中积累了丰富的与重金属铜耐性或富集相关的遗传变异,从而形成了对铜污染环境的高度适应。SRAP markers were employed to analysis the genetic diversity of a Commelina communis population（YL） growing on an ancient copper spoil heap in Tonglushan,Daye,with a population from nearby farmland（NT） used as a control.A total of 56 individuals from two populations were analyzed by using two primer pairs.24 loci with good resolvability were identified,of which 20 were polymorphic.A further analysis suggeted that the level of genetic diversity in YL population（H： 0.161,I： 0.231） is higher than that in NT population（H： 0.094,I： 0.145）.UPGMA analysis revealed that C.communis individuals from the same population are clustered together,which was further supported by the PCoA result.The present study revealed a significant difference in genetic variability between the two populations,which may be due to that the YL population located on the ancient copper spoil heap has gradually adapted to the copper contaminated environment during the long-time evolution.

关 键 词：鸭跖草 重金属污染 遗传多样性 遗传分化 SRAP标记 

分 类 号：Q14[生物学—生态学]