神经生长因子对新生大鼠缺氧缺血性脑损伤后脑组织Fas/FasL表达的影响  

Effects of NGF on Fas and FasL in brain tissue after HIBD in neonatal rats

作　　者：张素芬[1] 阴怀清[1] 常霞[1] 夏莉[1] 武师润[1] 阴崇娟[1] 

机构地区：[1]山西医科大学第一医院儿科,太原030001

出　　处：《中国药物与临床》2010年第4期391-393,共3页Chinese Remedies & Clinics

基　　金：太原市科技局科技兴市专项基金(0705-026)

摘　　要：目的探讨神经生长因子对新生大鼠缺氧缺血性脑损伤后(HIBD)脑组织Fas/FasL表达的影响。方法新生7d Wistar乳鼠120只随机分为3组,每组40只:A组(假手术组),B组(缺血缺氧组),C组[缺血缺氧+神经生长因子(NGF)]干预组。每组根据处死时间不同又分为5个亚组:6h组,12h组,24h组,48h组,72h组,每组各8只。建立HIBD模型,C组在缺血缺氧后即刻给予腹腔注射NGF(50μg·kg-1·d-1)1次/d,连续3d。各组在不同的时间点取脑组织,假手术组也在相应时间点取脑组织,并用免疫组织化学法测定Fas及FasL含量。结果①Fas的表达:A组脑组织在各个时间点均无或有少量表达;B组脑组织在各个时间点均有Fas的表达,且24～48h为表达高峰,以后逐渐下降;C组脑组织在各个时间点均有Fas的表达,但均较B组表达减少。3组各时间点比较差异均有统计学意义(P<0.01)。②FasL的表达:A组脑组织各个时间点均无表达或有弱表达;B组脑组织在各个时间点都有表达,且24～48h为表达高峰,以后逐渐下降;C组脑组织在各个时间点均有FasL的表达,但均较B组表达减少。3组各时间点比较差异均有统计学意义(P<0.01)。结论①新生大鼠HIBD后6,12,24,48,72h脑组织Fas及FasL表达增加,可能与脑缺氧缺血损伤后神经组织的凋亡有关;②NGF治疗后可明显减少新生大鼠HIBD后脑组织Fas及FasL的表达,从而减少脑组织缺氧缺血损伤后凋亡的发生。Objective To explore the effects of NGF on Fas and FasL in brain tissue after HIBD in neonatal rats. Methods A total of 120 seven-day-old Wistar rats were randomly divided into three groups （n=40 each）： A group （sham-operated control group）, B group （HIBD group）, and C group （HIBD ＋ NGF treatment group）. Each group were divided into five sub-groups （n=8 each） based on different time points after HIBD （6, 12, 24, 48 and 72 hours）. HIBD model was established. Instantly after HIBD, the rats in C group were given peritoneal NGF treatment （50 p,g.kg^-1. d^-1） once daily for three subsequent days. The animals were euthanized and harvested for brain tissues at different time points. Expressions of Fas and FasL in brain tissue were detected with immunohistochemistry. Results （1）Expression of Fas： Across all time points, expression of brain Fas appeared modest or negative in A group, and peaked at 24 to 48 hours followed by a gradual decline in B group, as did in C group but a little weaker as compared with B group （P〈 0.01）. （2）Expression of FasL： Across all time points, expression of brain FasL appeared modest or negative in A group, and peaked at 24 to 48 hours tbllowed by a gradual decline in B group, as did in C group but a little weaker as compared with B group （P〈0.01）. Conclusion （1）The enhanced expressions of Fas and FasL in brain tissues of neonatal rats with HIBD were found, possibly associated with apoptosis of nerve tissue after HIBD; （2）NGF may obviously reduced the brain expressions of Fas and FasL after HIBD, thereby protecting against HIBD-induced apoptosis of the brain tissue.

关 键 词：神经生长因子 缺氧缺血性 脑 抗原 CD95 

分 类 号：R722.121[医药卫生—儿科]