固定化谷氨酸脱羧酶的稳定性研究  

Study on the Stability of Immobilized Glutamic Decarboxylase on CM-CADB Resins

作　　者：吴国琪[1] 凌达仁[1] 王忱 王钒 宋国强[1] 

机构地区：[1]江苏石油化工学院化学工程系

出　　处：《江苏石油化工学院学报》1998年第4期1-3,共3页Journal of Jiangsu Institute of Petrochemical Technology

基　　金：江苏省科委资助

摘　　要：用羧甲基化的以Ｎ，Ｎ’－甲叉双丙烯酰胺交联的烯丙基葡聚糖（简称ＣＭ－ＣＡＤＢ）凝胶酶载体用树脂为新型载体，将谷氨酸脱羧酶固定在ＣＭ－ＣＡＤＢ树脂上。研究了固定化谷氨酸脱羧酶和自由酶与ｐＨ、温度的依存性，使用热稳定性和寿命。经测定谷氨酸脱羧酶经过固定化后，最适ｐＨ向碱性方向偏移０２个单位，ｐＨ为５２，最适温度为４８℃。固定化酶使用热稳定性比自由酶下降了５℃，但固定化酶的使用寿命得到显著改善，经测定固定化谷氨酸脱羧酶在４℃保存时，半衰期为１２７天。The immobilized L-glutamic decarboxylase on the CM-CADB (carboxymethyl-copolymer of allyl dextran and N,N'-methylene-bisacrylamide) ion exchange resin has been studied in this paper. The dependence of the activity of immobilized GDC and free enzyme on the pH, temperature was studied. The thermostability and the longevity were investigated. It is observed that the optimum pH(5 2) is 0 2 more than that of the free GDC(5 0). The optimum temperature is found to be around 48℃. The critical temperature of free GDC and immobilized GDC is respectively around 50℃and 45℃. The operational stability of immobilized GDC was examined. The half-life of the activity is 127 days, when the immobilized enzyme was kept in 4℃.

关 键 词：谷氨酸脱羧酶 固定化 稳定性 催化剂 

分 类 号：Q550.3[生物学—生物化学] O643.36[理学—物理化学]