低温胁迫下烟草海藻糖与海藻糖酶的动态关系研究  

Studies on the dynamic relation between trehalose and trehalase during cold-stress in tobacco

作　　者：杨军 易克[2,3] 周文辉 王勇 龚保国 

机构地区：[1]湖南省常德市产商品质量监督检验所,湖南常德415000 [2]湖南中烟工业有限责任公司技术中心,湖南长沙410125 [3]湖南农业大学生化与发酵实验室,湖南长沙410128

出　　处：《湖南文理学院学报（自然科学版）》2010年第1期31-34,共4页Journal of Hunan University of Arts and Science(Science and Technology)

摘　　要：研究了低温胁迫下烟草品种K326的海藻糖和海藻糖酶等指标的变化,结果表明:⑴低温锻炼、海藻糖和井冈霉素A处理均可引起烟草体内海藻糖含量增加,海藻糖酶活性降低,这种此消彼涨的关系反映了海藻糖可能是调节植物体抵抗逆境的一类应激物;⑵不同处理可使低温胁迫下烟草可溶性蛋白质代谢发生相应变化,SDS-PAGE结果证明三种处理均可能诱导低温胁迫相关蛋白的合成或表达增强.The changes of several physiological and biochemical indexes of tobacco K326 during cold-stress, which has been treated by cold-exercise, trehalose andjinggangmycin A, were assayed. The results suggested： （1）Three treatments all resulted that the contents of trehalose in tobacco increased and the activity of trehalase decreased, which proved that trehalose may be a product result of stress. （2）The contents of soluble protein took changes after different treatments, meanwhile, the results of SDS-PAGE showed that stress-relative protein may be induced or enhanced expression.

关 键 词：海藻糖 海藻糖酶 烟草 

分 类 号：Q559[生物学—生物化学]