楸树体细胞胚胎发生的研究  

Study on somatic embryogenesis of Catalpa bungei

作　　者：江荣翠[1] 彭方仁[1] 谭鹏鹏[1] 郝明灼[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2010年第2期15-18,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金："十一五"国家科技支撑计划(2006BAD24B08);江苏省高技术项目(BG2006319)

摘　　要：以楸树近成熟种子培养无菌苗,以无菌苗子叶和下胚轴为外植体进行体细胞胚胎发生研究,观察了体细胞胚胎的发育过程,分析了不同生长调节物质对楸树体胚发生的影响。结果表明:各培养基均能诱导出愈伤组织;1/2MS+0.01mg/LNAA+1.00mg/L6-BA可调整愈伤至胚性愈伤,是体细胞胚胎诱导频率最高的培养基;带少量愈伤的体胚可在1/2MS0和1/2MS+1.00mg/L6-BA+0.01mg/LNAA的培养基上增殖,且增殖系数较大。The process of somatic embryogenesis （SE） of Catalpa bungei and the effects of different growth regulators on SE were studied with explants of cotyledons and hypocotyls of seedlings. The seedlings were cultured from near matured seeds of C.bungei. The results showed that callus could be induced on each medium in this paper. Callus could be transferred to embryogenic callus on the medium of 1/2MS＋0.01 mg/L NAA＋1.00 mg/L 6-BA, on which the SE were induced with the highest frequency. The SE were able to proliferate on the medium of 1/2MS0 and 1/2MS＋1.00 mg/L 6-BA＋0.01 mg/L NAA with high multiplication coefficient.

关 键 词：楸树 愈伤组织 体细胞胚胎发生 

分 类 号：S722[农业科学—林木遗传育种] Q948[农业科学—林学]