激素和外植体类型对新疆雪莲愈伤组织诱导的影响  

Effect of Plant Hormones and Different Explants on Callus Induction of Saussurea involucrate

作　　者：庞红霞[1] 祝长青[1] 覃建兵[1] 

机构地区：[1]新疆师范大学生命科学与化学学院,乌鲁木齐830054

出　　处：《华中农业大学学报》2010年第2期131-134,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金(30960092;30960044);科技部援疆计划项目(200840102-39);新疆维吾尔自治区高校科研计划重点项目(XJEDU2007S27);新疆维吾尔自治区重点实验室开放课题(XJYS0203-2008-01);新疆师范大学研究生创新基金资助

摘　　要：以新疆雪莲叶片、叶柄和根为试验材料,研究了MS基本培养基附加不同质量浓度的植物激素对新疆雪莲愈伤组织诱导的影响。结果表明:单因素诱导中,NAA的诱导效果显著优于其它激素;以NAA为诱导激素,叶片与根,叶柄与根之间的诱导率存在极显著性差异,诱导效果:根>叶柄>叶片,最高诱导率分别为100%、98%、44%;NAA与6-BA激素组合对3种外植体愈伤组织的诱导中,叶片与叶柄诱导率之间存在极显著性差异,叶片、叶柄与根的诱导率之间存在显著性差异,诱导效果:叶片>叶柄>根,最高诱导率分别为98%、64%、23%。Using leaves,petioles and roots of Saussurea involucrate as explants,the effect of plant hormones on callus induction in MS medium was studied.The results showed that the medium containing NAA was better than other media.Comparing with whole explants,callus induction of leaves and roots,petioles and roots differed significantly.The inducing effect of roots,leaves and petioles was 100%,98% and 44%,respectively.Using the combination of NAA and 6-BA,callus induction of leaves,petioles and roots differs significantly as well.The induction effect of leaves,petioles and roots was 98%,64% and 23%,respectively.

关 键 词：新疆雪莲 愈伤组织 诱导 植物激素 外植体 

分 类 号：Q943.1[生物学—植物学]