不同固定液及染色方法对显示肥大细胞的影响  

Effect of Different Fixatives and Stainings on Presenting Mast Cells

作　　者：张高英[1] 杨宇[1] 罗广生 赵雅心[1] 彭克美[1] 刘华珍[1] 

机构地区：[1]华中农业大学动物医学院,武汉430070 [2]玉林出入境检验检疫局,玉林537000

出　　处：《华中农业大学学报》2010年第2期199-202,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30800808);华中农业大学科技创新项目(2007XCX010)资助

摘　　要：为寻求一种较好的显示肥大细胞的方法,采用硫堇染色法和硫堇-甲苯胺蓝染色法分别对用不同固定液固定的兔肠系膜中肥大细胞进行染色。结果显示:Carnoy氏液固定,硫堇染色可清楚显示肥大细胞,背景干扰小,阳性细胞多且可见肥大细胞的脱颗粒现象;硫堇-甲苯胺蓝法虽然也可显示肥大细胞,但背景干扰大,不利于对肥大细胞的观察;甲醛-乙醇固定,2种染色方法都可清楚地显示肥大细胞,但阳性细胞较少;中性甲醛则完全阻断肥大细胞的着色,2种染色方法都不能显示肥大细胞。兔肠系膜肥大细胞有沿血管分布的趋势。研究结果表明:Carnoy氏液及硫堇染液分别是兔肠系膜优良的固定液和染色液,可清楚显示肥大细胞。In the present study,rabbit mesentery was fixed in three different fixatives and stained with thionine and thionine-toluidine blue respectively in order to find a good method to display mast cells.The results showed that lots of positive mast cells with light background could be presented clearly by Carnoy fixative and thionine staining.In addition,degranulation of some mast cells could be observed clearly.However,it was difficult to observe positive mast cells by Carnoy fixative and thionine-toluidine blue staining because of high interferential background.Only a little positive mast cells could be observed through formaldehyde-alcohol fixative and the two staining methods.Neutral formalin blocked the staining of mast cells completely,for neither of the staining methods could display mast cells.Most of the positive mast cells were distributed along the blood vessels.The results demonstrated that Carnoy and thionine were superordinary fixative and staining solution for clearly displaying mast cells of rabbit mesentery,respectively.

关 键 词：肥大细胞 固定液 染色 兔 肠系膜 

分 类 号：S852.16[农业科学—基础兽医学]