1株黄颡鱼肠道益生菌的筛选与鉴定  

Screening and Identification of a Potential Probiotic from Pelteobagrus fulvidraco Intestine

作　　者：孟小亮[1] 陈昌福[1] 高宇[1] 贺中华[1] 田甜[1] 刘振兴[1] 

机构地区：[1]华中农业大学水产学院,武汉430070

出　　处：《华中农业大学学报》2010年第2期208-212,共5页Journal of Huazhong Agricultural University

基　　金：国家"973"项目(2009CB118700);农业公益性行业科研专项经费项目(200803013);农业部疫情监测项目(2130108)资助

摘　　要：从正常养殖的黄颡鱼肠道中分离得到218株细菌,通过产酶能力测试,对常见病原菌体外拮抗作用以及对抗生素敏感性试验筛选出1株益生菌HS140菌株。通过测定HS140菌株对黄颡鱼的致病性,并结合形态观察、生理生化试验和16SrRNA部分序列分析,对HS140菌株进行了分类鉴定。结果显示:HS140菌株具有很强的分泌蛋白酶、脂肪酶和淀粉酶的能力,能够抑制温和气单胞菌、迟缓爱德华菌和鳗弧菌的生长。HS140菌株对氨苄青霉素、磺胺异恶唑、先锋霉素VI等3种抗生素产生耐药性,对另外13种抗生素均敏感或中度敏感。急性毒性试验表明,浓度为1.0×108cfu/mL的HS140菌悬液对黄颡鱼没有致病性。HS140菌株为革兰氏阳性可动杆菌,16SrRNA的部分序列分析显示HS140菌株与地衣芽孢杆菌(Bacillus licheniformis)具有99.3%的相似性,系统发育树上HS140菌株与地衣芽孢杆菌(FJ435674)聚为同一分支,结合形态观察和生理生化试验结果最终鉴定HS140菌株为B.licheni formis。Two hundred and eighteen bacteria strains isolated from intestinal tract of cultured Pelteobagrus fulvidraco were used for testing the producing zymogenicities of protease,lipase and amylase,the antagonistic against five common pathogenic bacterias and the antibiotic sensitivity.The toxicity of strain HS140 screened as potential probiotic for Pelteobagrus fulvidraco was evaluated.HS140 was identified according to its morphological,physiological and biochemical characteristics as well as homology analysis of 16S rRNA sequences.The results indicated that HS140 had high ectoenzyme-producing ability and could produce chemical substances inhibitor to Edwardsiella tarda,Aeromonas sobria and Vibrio anguillarum.HS140 was resistant to ampicillin,sulfamethoxazole,VI cefradine,but sensitive or medium sensitive to the other 13 kinds of antibiotics.It was safe for Pelteobagrus fulvidraco when they were challenged by a high immersion concentration of HS140 at 108 cfu/mL.Morphology observation showed that HS140 was gram-positive,rod,motile.The results revealed that strain HS140 comprised sequences more related to Bacillus licheniformis（99.3%） from partial 16S rRNA sequences.The identification showed that strain HS140 was Bacillus licheniformis by the way of morphology,biochemistry characteristic and 16S rRNA sequence homology analysis.

关 键 词：黄颡鱼 地衣芽孢杆菌 筛选 鉴定 

分 类 号：Q959.46[生物学—动物学]