氧化苦参碱对肺纤维化大鼠肺内Th1/Th2平衡的影响  

Effects of Oxymatrine on Balance of Th1/Th2 in Rats with Pulmonary Fibrosis

作　　者：徐佳波[1] 李燕芹[1] 刘斌[1] 秦慧[1] 邹静[1] 

机构地区：[1]上海交通大学附属仁济医院呼吸科,上海200127

出　　处：《上海中医药大学学报》2010年第2期53-57,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市科委科研基金资助项目(06411963)

摘　　要：目的:观察博莱霉素诱导的大鼠肺纤维化肺内Th1/Th2状态,以及氧化苦参碱(OMT)对肺纤维化大鼠的干预作用。方法:气管内注射博来霉素制作大鼠肺纤维化模型。将72只雄性SD大鼠分组为正常对照组、模型组、氧化苦参碱治疗组。治疗组于造模后第2天起予腹腔注射氧化苦参碱注射液,直至处死。每组在造模后第3、7、14、28天随机处死6只,观察肺组织病理变化,计算机图形自动分析系统(C IAS)评估肺纤维化程度;检测肺组织羟脯氨酸(HYP)含量及肺组织匀浆γ-干扰素(IFN-γ)及白介素-4(IL-4)。结果:与正常组比较,模型组表现为明显肺泡炎,炎性细胞浸润、严重肺纤维化。治疗组与模型组比较,肺泡炎和肺间质的纤维胶原组织增生均减轻(P<0.05)。模型组第14、28天肺HYP含量高于同期正常组(P<0.05);治疗组第28天肺HYP含量明显低于同期模型组(P<0.05)。与正常组比较,模型组IFN-γ均降低(P<0.05),但治疗组各时间点IFN-γ水平较模型组升高(P<0.05),且与正常组比较无统计学差异(P>0.05)。正常组、治疗组和模型组各时间点IL-4含量均无统计学差异(P>0.05)。结论:博莱霉素肺纤维化大鼠体内存在Th1反应不足现象,氧化苦参碱能减轻博莱霉素导致的大鼠肺纤维化程度,其可能与纠正肺纤维化大鼠Th1/Th2的失衡有关。Objective： To observe Th1/Th2 in rats with pulmonary fibrosis induced by bleomycin and investigate the effects of oxymatrine on pulmonary fibrosis in rats.Methods： The model of pulmonary fibrosis in rats was established by intratracheal perfusion of bleomycin.A total of 72 male SD rats were randomly divided into normal control group,model group and oxymatrine treated group.Since the second day of model establishment,the rats in treatment group were given intraperitoneal injection of Oxymatrine until to be sacrificed.Six rats from each group were randomly sacrificed on days 3,7,14,28 to observe the pathological changes in lung tissues.The degree of pulmonary fibrosis was assessed by computer image analysis system（CIAS）.The hydroxyproline（HYP）,interferon-γ（IFN-γ） and interleukin-4（IL-4） in lung tissue were also detected.Results： Compared with the normal group,there were marked alveolitis,inflammatory cell infiltration and lung fibrosis in the model group.Compared with the model group,the alveolitis and collagen deposition were alleviated in treatment group（P 0.05）.The HYP of model group was higher than that of normal group on 14th and 28th dags（P0.05）.The HYP of treatment group was lower than that of model group on day 28th（P0.05）.The concentration of IFN-γ in lung tissue of model group was significantly lower than that of normal group（P0.05）.The IFN-γ concentration of treatment group was higher than that of model group（P0.05）,and there was no significant difference between treatment group and normal group（P0.05）.There was no significant difference of the IL-4 concentration between the three groups（P0.05）.Conclusion： There is a hypo-response of Th1 in rats with pulmonary fibrosis,and oxymatrine can improve the pulmonary fibrosis,whose mechanisms may relate to correcting the imbalance of Th1/Th2.

关 键 词：肺纤维化 氧化苦参碱 TH1/TH2 

分 类 号：R285.5[医药卫生—中药学] R563.9[医药卫生—中医学]