SA、H_2O_2和6-BA预处理对沟叶结缕草耐寒性的影响  

Effect of pre-treatments with SA、H_2O_2 and 6-BA on chilling tolerance in Zoysia matrella

作　　者：王艳[1] 李建龙[1] 姜涛 邓蕾[1] 

机构地区：[1]南京大学生命科学院,江苏南京210093 [2]连云港曲情生物科学技术有限公司,江苏连云港222000

出　　处：《草业学报》2010年第2期76-81,共6页Acta Prataculturae Sinica

基　　金：国家高科技研究发展计划(863计划)项目(2007AA10Z231);中国博士后科学基金面上资助项目(200904501077)资助

摘　　要：通过对沟叶结缕草进行叶面喷施信号化合物SA、H2O2和6-BA,研究了预处理对沟叶结缕草耐寒性的影响。结果表明,SA、H2O2和6-BA预处理可以有效控制或者防止结缕草叶片中活性氧的积累和氧化伤害的发生,从而提高其抗寒性;预处理植株中POD、CAT和APX活性在胁迫过程中显著高于对照,冷胁迫本身可以诱导SOD的活性避免O2-的积累,GR在抗寒胁迫过程中可能起到负反馈的作用,抗氧化酶活性升高与沟叶结缕草耐寒性的获得密切相关;在这3种信号化合物中,SA预处理植株中SOD,CAT和GR活性高于对照和其他化合物预处理植株,并且具有最小的MDA浓度,因此,SA对于提高沟叶结缕草的耐寒性最有效。Following leaf application of signaling compounds such as SA,H2O2,and 6-BA,the effect of three pretreatments on the chilling tolerance in manila grass was studied.SA,H2O2,and 6-BA pretreatments improved the chilling tolerance of manila grass by controlling and preventing the accumulation of reactive oxygen species and the occurrence of oxidative damage.POD,CAT,and APX activities in pretreatments were significantly higher than in controls,and SOD activities were increased by chilling itself.GR may regulate the oxidant-reduction status as feedback,indicating that these antioxidative enzymes were closely related to the improvement of cold resistance in manila grass.SA induced more SOD,CAT,and GR activities than other compounds,suggesting SA may be the most effective of the four compounds for improving the chilling tolerance of manila grass.

关 键 词：暖季型草坪草 低温胁迫 信号化合物 氧化伤害 抗氧化酶 

分 类 号：S688.4[农业科学—观赏园艺]