硅酸盐细菌和苏云金芽孢杆菌原生质体融合  

INTERSPECITIC PROTOPLAST FUSION BETWEEN BACILLUS MUCILAGINESUS W9-12 AND BACILLUS THURINGESIS Bt

作　　者：唐宝英[1] 朱晓慧[1] 刘佳[1] 

机构地区：[1]江苏省微生物研究所,无锡214063

出　　处：《生物技术》1998年第5期19-21,31,共4页Biotechnology

摘　　要：具有解钾活性的硅酸盐细菌──胶质芽孢杆菌（R．mucilaginesus）W9－12与具有杀虫活性的苏云金芽孢杆菌（B．thuringensis）Bt179208原生质体质进行了融合。W9－12原生质体形成率为99．7％．再生率为24．9％，Bt179208原生质体形成率为99．9％，再生率为35％。以PEG为助融剂进行原生质体融合．得到56个融合子．融合率为5．92×10－6。融合子在双抗选择培养基上连续传代10次，得到12个稳定的融合子，生物测定表明，融合子既具有一定的杀虫活性又具有一定的解钾活性。The results of the interspecific protoplast fusion between B. mucilag tnosss W9 - l2 which has decomposing potassium activity, and B. thuringensis Bt 179208 which has pesticide ability, were reparted- High frequency of protoplast formation and regeneration was obtained with 12h activated W9 - 12 treated by 2mg/ml lysozyme. Using 40% PEGand 5% nascent Ca2+ to treat the parential protoplast mixture for 10min at 37℃, 12 stable fusants were obtained. Biological tests show that they have both pesticide ability and decomposing potassium activity.

关 键 词：硅酸盐细菌 苏云金芽孢杆菌 原生质体融合 

分 类 号：Q939.124[生物学—微生物学] Q933