真菌接合孢子囊的形成及特征观察实验的改进探索  

Improvement of character observation experiment of formation process of zygosporangium in Fungi and an analysis on its teaching effect

作　　者：徐德强[1] 王英明[1] 肖义平[1] 

机构地区：[1]复旦大学生命科学学院

出　　处：《实验技术与管理》2010年第3期36-38,共3页Experimental Technology and Management

基　　金：国家基础科学人才培养基金项目(J0630643)

摘　　要：真菌接合孢子囊的形成及特征观察是微生物学实验教学内容之一。采用蓝色犁头霉＂＋＂和＂-＂菌株为实验菌种（取代以往常用的匍枝根霉）,并以＂八＂字型划线接种,同时拍摄了该菌种形成接合孢子囊的5个生长阶段典型特征的显微镜照片用于实验教学,不但减轻了以往匍枝根霉孢囊孢子等易污染实验室环境的现象,也避免了该菌种对培养温度较敏感、常易引起实验结果不理想的状况。同时使学生知识学得更活、更扎实,并对培养学生创新意识也具有一定的指导意义,因而提高了该实验教学的效果,受到学生的欢迎。The character observation of formation process of zygosporangium in Fungi is one of teaching contents in microbiological experiments.The ＂＋＂ and ＂-＂ strains of Absidia coerulea were selected as the experiment species,instead of Rhizopus stolonifer,frequently used before.Then,Fungi were inoculated by streaking plates according to the shape of ＂八＂.Some micrographs were taken to serve the experiment teaching.These micrographs described typical characters of 5 growth phases through which this species formed zygosporangium.This will decrease the pollution phenomenon caused by using sporangiospore of Rhizopus stolonifer,and also avoid the situation in which this species is hypersensitive to cultural temperature,resulting in bad experiment result.Meanwhile,this will give students a live study and make them have great basic knowledge.In addition,this is helpful for developing students＇ innovative consciousness.This process has received much welcome from students because of enhancing experiment teaching effect.

关 键 词：真菌 蓝色犁头霉 接合孢子囊 实验 

分 类 号：Q-331[生物学]