太原地区丙型肝炎病毒基因分型及其临床意义  

Genetypes and its clinical significance of hepatitis C virus in Taiyuan region

作　　者：王琰[1] 朱新宇[2] 王霞[1] 王东[1] 

机构地区：[1]山西省太原市传染病医院,030012 [2]山西医科大学第一附属医院感染病科

出　　处：《实用肝脏病杂志》2010年第2期109-111,共3页Journal of Practical Hepatology

摘　　要：目的了解本地区HCV基因型分布,探讨HCV基因分型的临床意义。方法用RT-nested-PCR方法扩增62例丙型肝炎患者血清HCV5’ UTR区,限制性内切酶HaeⅢ、Bsh1236Ⅰ进行双酶切得到不同长度基因片段,分析结果,确定基因型。结果62例丙型肝炎患者中,其中1b型43例,2a型9例,1b/2a混合型5例,1a型1例,3a型1例,未分型3例。基因1b型患者肝功能、肝脏脂肪变性均较非1b型严重。结论本地区丙型肝炎以基因1b型为主,且该型患者临床病情较其它型严重。Objective To explore genotype distribution and clinical significance of HCV in Taiyuan region.Methods To amplify 5＇UTR of HCV RNA in 62 patients using RT-nested-PCR,to cut 5＇UTR fragment into different length clips by using incision enzyme HaeⅢ and Bsh1236Ⅰ.Results In 62 patients with hepatitis C,43 cases were infected with genotype 1b,9 cases were infected with genotype 2a,5 cases was 1b/2a mixed-type,1 case was type 1a,1 case was type 3a,and 3 cases were not tested.The patients with 1b genotype were more serious than the patients with non-genotype 1b in the hepatic function and liver fatty degeneration.Conclusion In this locality hepatitis C were dominated by genotype 1b,and the pathogenetic condition of genotype 1b patients more serious than non-genotype 1b patients.HCV genotypes 1b accounted for 69.4% of HCV infections in patients from this geographical region.HCV genotype 1b was associated with more serious clinical performance.

关 键 词：丙型肝炎病毒 基因分型 基因亚型 限制性片段长度多态性分析法 

分 类 号：R512.63[医药卫生—内科学]