小鼠胸腺上皮细胞系MTEC1产生30kD的MCP-1类趋化因子  

30KD MCP-1 PROTEIN PRODUCED BY MOUSE THYMIC EPITHELIAL CELL LINE MTEC1

作　　者：刘祝公[1] 陈慰峰[1] Jo Van Damme 

机构地区：[1]北京医科大学免疫学系,北京100083 [2]Rega Institute,Katholieke University Leuven,Belgium

出　　处：《实验生物学报》1998年第3期245-250,共6页Acta Biologiae Experimentalis Sinica

基　　金：欧共体基金Cl1*-CT92-0066

摘　　要：髓质型小鼠胸腺上皮细胞系MTEC1自发分泌趋化因子(Chemokines),细胞培养上清先经CPG珠(Controlled-pore glass beads)初步浓缩纯化,再依次经肝素-Sepharose柱亲和层析、阳离子交换FPLC及RP-HPLC分离纯化,得到了一个30kD的蛋白质分子,对淋巴细胞和单核巨噬细胞均有趋化活性。此蛋白分子N端封闭,因而先经甲酸水解得到蛋白片段,分析了其中24个氨基酸的序列,发现其与小鼠MCP-1/JE(Monocyte Chemoattractant Pro-tein-1)完全相同。以上结果说明,MTEC1产生的是mMCP-1/JE类趋化因子。A medullary type mouse thymic epithelial cell line MTECl can produce chemokines. Crude culture supernant of MTECl cells was concentrated by controlled-pore glass beads. One of these chemotactic proteins was isolated from MTECl supernatant and purified to homogeneity by heparin-Sepharose affinity chromatography, cation-exchange FPLC and Reverse Phase-HPLC. The chemotactic factor for both lymphocytes and monocytes was identified as a 30 kD protein by SDS-PAGE analysis under reducing conditions. After cleavage of the NH2-terminally blocked protein with formic acid, the amino acid sequence of the internal fragment was analyzed and found to be identical to the amino acid sequence of mouse MCP-1/JE. The protein was hence identified as a glycosylated MCP-1/JE-like chemokine secreted by thymic epithelial cells. The characterization of chemokines produced by thymic stromal cells will benefit the analysis of the mechanism of thymus homing and the finding of new chemokines.

关 键 词：趋化因子 胸腺革质细胞 MCP-1 糖基化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]