中肺合剂对小鼠Lewis肺癌肿瘤组织Survivin Caspase-3表达的影响  

The Effects of Zhongfei Mixture on the Expression of Survivin and Caspase-3 in Tumor Tissues from the Mice Bearing Lewis Lung Carcinoma

作　　者：周晓芳[1] 林能明[1,2] 徐利[2] 韩江敏[1] 张会从[2] 张如松[2] 

机构地区：[1]浙江省肿瘤医院,浙江杭州310022 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《中华中医药学刊》2010年第4期768-770,I0002,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划资助项目(2007CA098)

摘　　要：目的:观察中肺合剂对小鼠Lewis肺癌肿瘤组织survivin、caspase-3基因表达的影响。方法:取C57BL/6小鼠50只,接种Lewis肺癌瘤株后,随机分阴性对照组(0.9%生理盐水),中肺合剂低、中、高剂量组(12.7g·kg-1、25.4g·kg-1、50.8g·kg-1),阳性对照组(环磷酰胺组,30mg·kg-1),每组10只。观察12天停药。无菌条件下取肿瘤组织,采用EnVision免疫组化方法测定中肺合剂对小鼠Lewis肺癌组织survivin、caspase-3蛋白表达的影响。结果:中肺合剂低、中、高给药组能不同程度下调Lewis肺癌小鼠肿瘤组织survivin蛋白的表达并上调caspase-3蛋白的表达,且均以中剂量组最显著,与生理盐水组相比统计学上有非常显著差异(P<0.01)。结论:中肺合剂可通过下调survivin表达和上调caspase-3表达,从而促进肿瘤细胞凋亡,抑制肿瘤组织生长。Objective： To observe the effects of Zhongfei Mixture（ZM） on the expression of survivin and caspase -3 in tumor tissues from the mice bearing Lewis lung carcinoma. Methods： Fifty C5？BL/6 mice bearing Lewis lung carcinoma were divided into 5 groups ：the saline control group, the low, mid and high - dose groups of ZM and Cyclophosphamide （CTX） group. Tumor tissues were isolated after 12 days followed by the expression det^ctiun of survlvin and easpase -3 in tumor tissues by EnVision two - step immuno - histochemistry staining. Results ： ZM could down - regulate the expres- sion level of survivin and up - regulating caspase - 3 in tumor tissues from the mice bearing Lewis lung carcinoma. Com- pared with saline control group, the effect of the mid - dose group was the most prominent and showed signitlcanfly differ- ent in statistics（ P 〈 0. 01 ）. Conclusion： ZM could inhibit proliferation and induce apoptosis in tumor cells from the mice bearing Lewis lung carcinoma. The possible mechanism is associated with down - regulating the expression level of survivin and up - regulating caspase - 3 in tumor tissues respectively.

关 键 词：中肺合荆 LEWIS肺癌 ENVISION法 SURVIVIN caspase-3 

分 类 号：R285.5[医药卫生—中药学]