桂枝茯苓胶囊对体外人宫颈癌Hela细胞抑制作用及机理研究  

Study of Inhibition and Mechanism on Hela of Guizhi Fuling Capsule in Vitro

作　　者：黄燕芬[1] 董改霞[1] 柴慧[1] 袁小凤[1] 

机构地区：[1]浙江中医药大学生命科学学院,浙江杭州310053

出　　处：《中华中医药学刊》2010年第4期774-777,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅基金资助项目(20060721)

摘　　要：目的:研究桂枝茯苓胶囊对体外培养人宫颈癌Hela细胞的增殖抑制作用和作用机理的初步研究。方法:MTT比色法观察桂枝茯苓胶囊对人宫颈癌Hela细胞增殖的抑制作用;免疫组化检测Fas、Fas-L、Bcl-2基因的表达;western-blot和RT-PCR检测对人宫颈癌Hela细胞Caspase-3蛋白和Caspase-3MRNA的表达影响;DNA-Ladder检测凋亡条带。结果:桂枝茯苓胶囊对人宫颈癌Hela细胞有明显的抑制作用(P<0.01);桂枝茯苓胶囊能促进Fas、Fas-L表达,抑制Bcl-2的表达;能增加Hela细胞Capase-3的蛋白表达;琼脂糖凝胶电泳中呈现典型的"阶梯状"图谱。结论:桂枝茯苓胶囊有体外抗肿瘤作用,其机制可能是通过促进Fas、Fas-L表达、抑制bcl-2的表达;增加Capase-3的表达实现的。Objective： To study the effects on anti-proliferation and its mechanism on the Hela of Guizhifuling Capsule. Methods： The inhibitory effect on Hela cell proliferation was assayed by MTT. Fas,FasL,Bcl-2 gene＇s expression was detected by Immunohistochemistry. caspase-3 MRNA and protein were detected by Western-blot and RT-PCR. Results： Guizhifuling Capsule have obvious effect on anti-proliferation of Hela cells （P〈0.01）. Guizhifuling Capsule can promote Fas and Fas –L to express and inhibit expression of Bcl-2. Guizhifuling Capsules can reduce expression of capase-3 protein and promote capase-3 MRNA in Hela. Typ icalDNA ladder appeared in agarose gel electrophoresis.Conclusion： Guizhifuling Capsule can inhibit the inhibit proliferation of Hela cells. Promoting the expression of caspase-3, promoting the expression of Fas expression and inhibiting the expression of Bcl-2 may be the mechanisms of its anti-tumor.

关 键 词：桂枝茯苓胶囊 宫颈癌Hela细腻 MTT western—blot 免疫组化 

分 类 号：R285.5[医药卫生—中药学]