软坚消瘿汤治疗实验性自身免疫性甲状腺炎疗效机制研究  

Effect Mechanism of Ruanjian Xiaoying Decoction on Experimental Autoimmune Thyroiditis

作　　者：张兰[1] 方振伟[1] 

机构地区：[1]辽宁中医药大学第一附属医院,辽宁沈阳100032

出　　处：《中华中医药学刊》2010年第4期843-845,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅资助项目(20060543)

摘　　要：目的:观察软坚消瘿汤对实验性自身免疫性甲状腺炎(EAT)大鼠的自身抗体、白介素-1、4、6及肿瘤坏死因子-α和甲状腺形态学的影响,从多靶点探讨其作用机制。方法:运用甲状腺球蛋白与弗氏佐剂混合免疫注射法,联合饮用高碘水模拟大鼠实验性自身免疫性甲状腺炎。酶联免疫法检测甲状腺抗体、白介素-1、4、6及肿瘤坏死因子-α水平,HE染色法观察大鼠甲状腺病理改变。结果:软坚消瘿汤能显著降低EAT大鼠自身抗体及相关细胞因子水平,减轻甲状腺滤泡损伤。结论:软坚消瘿汤通过降低EAT血清中自身抗体及细胞因子水平起到减轻甲状腺病理损伤的作用。Objective：To evaluate the therapeutic effect of Xiaoying Ruanjian Decoction on experimental autoimmune thyroiditis （EAT） from the levels of autoantibodies, interleukin-1,4,6 and tumor necrosis factor-α and the thyroid morphology of EAT rats.To Investigate the effect Mechanism from multiple target point.Methods：An animal model of experimental autoimmune thyroiditis （ EAT） in rats was developed by injecting thyroglobulin mixed with Freund＇s adjuvant immunization and drinking Periodate water. To detect the levels of autoantibodies, interleukin-1,4,6 and tumor necrosis factor-α with Enzyme-linked immunosorbent assay.To observe the pathological change in the thyroid of rat with HE staining.Results：Ruanjian Xiaoying Decoction significantly reduces the levels of autoantibody, cytokines and the damage of thyroid follicular.Conclusion：Ruanjian Xiaoying Decoction alleviates the pathological injury of thyroid by the suppression of relative expressions of antibodies and cytokines in serum levels.

关 键 词：软坚消瘿汤 实验性自身免疫性甲状腺炎 血清抗体 细胞因子 中医实验医学 

分 类 号：R285.5[医药卫生—中药学]