亚麻木酚素对糖尿病小鼠肾脏保护作用及机制  

Study on the Protective Effect of Flaxseed Lignan Extract on Kidney in Diabetic Mice

作　　者：洪兵[1] 徐海娥[2] 胡文[1] 李莉[3] 徐晓文[1] 徐伟[1] 马绍刚[1] 俞伟男[1] 

机构地区：[1]淮安市第二人民医院内分泌科,江苏淮安223002 [2]南京医科大学第二附属医院营养科,江苏南京210011 [3]江苏省疾病预防控制中心,江苏南京210009

出　　处：《中华中医药学刊》2010年第4期856-859,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：淮安市科技局基金资助项目(HAS06045)

摘　　要：目的:观察亚麻木酚素提取物(FLE)对糖尿病小鼠早期肾损伤的影响并探讨其作用机制。方法:雄性ICR小鼠分6组:正常对照组,糖尿病模型对照组,糖尿病模型亚麻木酚素提取物[600mg/(kg·d)、1.2g/(kg·d)、3.6g/(kg·d)]治疗组,糖尿病小鼠盐酸二甲双胍[100mg/(kg·d)]治疗组。连续灌胃8周,实验结束后比较各组体重、肾重、肾重/体重、24h尿白蛋白排泄率、肾组织MDA含量及肾组织SOD、GSH-PX活性,HE染色分析肾小球病理形态学改变,综合评价亚麻木酚素提取物对肾脏的保护作用。结果:与糖尿病模型对照组比较,亚麻木酚素提取物中、高剂量组的肾重、24h尿白蛋白排泄率显著降低(P<0.05),MDA含量降低(P<0.05),亚麻木酚素提取物各剂量组的肾脏组织SOD、GSH-Px活性增加(P<0.05)。结论:亚麻木酚素提取物能减轻糖尿病小鼠肾脏损伤,对肾脏有保护作用,其机制与其减轻肾脏氧化应激有关。Objective： To investigate the protective effect of of Flaxseed Lignan Extract （FLE） on kidney in diaetic mice. Methods ： ICR mice were randomly divided into 6 groups ： normal mice control, STZ diabetic mice control, STZ di- abetic mice fed with FLE at dose of [600mg/（kg ·d）, 1.2g/（kg · d）, 3.6g/（kg·d） ], and STZ diabetic mice fed with mefformin. The above components were administed intragastrically for 8 weeks. Then their blood glucose, kidney weight to body weight, 24 - hour urinary protein,the content of MDA and the activities of SOD, GSH - Px of kidney were detected. Glomerular structure of kidney were examined by light microscopy. Results： The kidney weight to body weight, 24 - hour urinary protein in high,middle dose of FLE groups were significantly lower than those in diabetic control group （ P 〈 0.05）. The content of MDA in all dose of FLE groups were significantly lower than those in diabetic control group （ P 〈0.05）. And the activities of SOD, GSH - Px in high, middle dose of FLE groups were significantly higher than those in diabetic control group （ P 〈 O. 05 ）. Conclusion： Flaxseed Lignan Extract can ameliorate the kidney damage, which has a protective effect maybe by inhibiting the oxidative stress of kidney.

关 键 词：木酚素 糖尿病 肾脏 氧化应激 

分 类 号：R285.5[医药卫生—中药学]