强直性脊柱炎患者附着端炎与外周血淋巴细胞趋化因子受体5及血浆白细胞介素1β的相关性  

Expression of Chemokine Receptor 5 and Interleukin-1β in Enthesitis of Ankylosing Spondylitis

作　　者：赵嘉英[1] 赵阴环[2] 

机构地区：[1]秦皇岛市第一医院风湿免疫科,河北秦皇岛066000 [2]哈尔滨医科大学附属第二医院风湿科,哈尔滨150000

出　　处：《中华临床免疫和变态反应杂志》2010年第1期22-25,共4页Chinese Journal of Allergy & Clinical Immunology

摘　　要：目的探讨强直性脊柱炎(ankylosing spondylitis,AS)患者附着端炎发生的可能机制。方法采用流式细胞仪检测36例AS患者外周血淋巴细胞趋化因子受体5(chemokine receptor 5,CCR5)的表达,同时用酶联免疫吸附法检测其血浆白细胞介素1β(interleukin-1β,IL-1β)的表达,对比附着端炎指数MASES﹥0患者与MASES=0患者的CCR5及IL-1β表达的差异。并对附着端炎指数与趋化因子受体CCR5及IL-1β表达的相关性进行分析。结果(1)MASES﹥0组C反应蛋白(C-reactive protein,CRP)为(42.54±42.59)mg/L,明显高于MASES=0组的(18.52±18.43)mg/L(P=0.026);MASES﹥0组CCR5为(24.70±9.39)%,高于MASES=0组的(16.57±13.32)%,但两组比较差异无显著性意义(P=0.119);MASES﹥0组白细胞介素1β(interleukin 1β,IL-1β)为(2.98±1.74)pg/ml,明显高于MASES=0组的(1.22±0.73)pg/ml(P<0.05)。(2)附着端炎指数MASES与CCR5和IL-1β成正相关(分别r=0.684,P<0.001;r=0.448,P<0.05)。结论CCR5、IL-1β与AS附着端炎发生的分子免疫学机制有关。Objective To study the possible molecular mechanism of enthesitis in ankylosing spondylitis （AS）. Methods The levels of lymphocytes CCR5 expression were investigated by flow cytometry in 36 patients with AS. The level of IL-1β was detected by ELISA in these patients. We analyzed the relationship between Maastricht Ankylosing Spondylitis Enthese Score （MASES）, CCR5 and IL-1β. Results The level of IL-1β in MASES﹥0 group [（2.98±1.74） pg/ml] was significantly higher than MASES=0 group [（1.22±0.73） pg/ml] （P〈0.05）. MASES had a positive correlation with CCR5 and IL-1β （r = 0.684, P〈0.001; r = 0.448, P〈0.05）. Conclusion CCR5 and IL-1β may be involved in the molecular immunology mechanisms of enthesitis of AS patients.

关 键 词：强直性脊柱炎 附着端炎 趋化因子受体5 白细胞介素1Β 

分 类 号：R593.23[医药卫生—内科学] R392.11[医药卫生—临床医学]