免疫胶体金试纸条检测肉鸡感染H9N2-AIV后NS1抗体消长规律  

Situation of NS1 antibodies in H9N2 AIV-infected broilers idntified by immunochromatographic strip

作　　者：张文泽[1] 崔卫涛[1] 彭伏虎 肖运才[1] 刘梅[1] 王喜亮[1] 胡思顺[1] 毕丁仁[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]湖北省动物疫病预防控制中心,湖北武汉430064

出　　处：《中国兽医学报》2010年第3期308-311,共4页Chinese Journal of Veterinary Science

基　　金：湖北省科技攻关重大专项(2006AA202A05);华中农业大学科研启动经费专项(52204-07006)

摘　　要：禽流感病毒非结构蛋白(AIV-NS1)抗体检测可作为禽流感病毒感染的诊断标签。在禽流感NS1抗体鉴别诊断试纸条研制成功的基础上,本试验采用人工感染的方法,探讨了H9亚型禽流感病毒感染肉鸡体内NS1特异性抗体的消长规律。结果表明,肉鸡感染后3d开始检测出NS1抗体,至11d阳性率达89%,然后快速下降,21d检出率下降至10%,26d后完全消失,NS1抗体阳性率高低与感染鸡发病及死亡率高低明显一致。而对照组和疫苗免疫组在整个试验过程中NS1抗体检测均呈阴性。感染组血凝抑制抗体出现早于疫苗免疫组,但二者效价持续上升的结果类似。通过肉鸡人工感染试验,揭示了禽流感病毒感染后肉鸡体内NS1抗体消长情况,同时也证实本研究室研制的AIV-NS1抗体检测试纸条,不仅可以从患有呼吸道感染的病鸡中快速诊断出禽流感病毒感染,而且也可以鉴别禽流感疫苗免疫与病毒感染的鸡群。The domestic poultry infected by avian influenza could not be identified by traditional serum tests but marked by nonstructural protein one （NS1）.Based on the successful development of immunochromatographic strip of differentiating chickens vaccinated and AIV-infected,we investigated the kinefics of the antibodies against NS1 in broilers experimently infected by avian influenza virus （AIV）.Results showed that in virus-infected chickens the detectable antibodies to NS1 was first detected at 3 days after infection,and declined rapidly after 11days （positive rate up to 89%） and disappeared until 26 days post-infection,whilst the vaccinated-chickens and PBS-control chickens were negative in NS1-Abs detection.The situation of NS1-Abs was strongly compatible to the rates of morbidity and mortality of the broilers infected by H9N2-AIV.Though HI-Ab induced in AIV-infected-chickens was earlier than that of vaccinated-chickens,the situation of the groups was similar to each other.Through the experimental infection tests,the situation of the antibodies against NS1 of AIV was first understood and discovered,furthermore it testified that the immunochromatographic strip for detecting NS1-Ab was not only feasible to identify whether the respiratory infecting chickens was infected with AIV,but also to distinguish AIV-infected-chicken from vaccinated-chicken.

关 键 词：免疫层析试纸条 H9N2亚型禽流感 非结构蛋白 肉鸡 

分 类 号：S852.65[农业科学—基础兽医学]