副猪嗜血杆菌ERIC-PCR指纹图谱多样性研究  

Characterization of the diversity of Haemophilus parasuis field isolates by use of ERIC-PCR

作　　者：贾爱卿[1,2] 李春玲[1,2] 王贵平[1,2] 杨冬霞[1,2] 

机构地区：[1]广东省农业科学院兽医研究所,广东广州510640 [2]广东省兽医公共卫生公共实验室,广东广州510640

出　　处：《中国兽医学报》2010年第3期337-339,383,共4页Chinese Journal of Veterinary Science

基　　金：广东省农业攻关项目(2007A020300005-11)

摘　　要：建立了副猪嗜血杆菌ERIC-PCR分型技术,并应用于临床分离菌株的流行病学调查。试验结果表明,47株副猪嗜血杆菌产生28种不同的指纹图谱,分离自同一猪场和相同血清型的菌株表现出不同的指纹图谱,不能进行血清分型的副猪嗜血杆菌应用该方法可得到充分区分,证实副猪嗜血杆菌ERIC指纹图谱存在丰富的多样性,ERIC-PCR方法是副猪嗜血杆菌流行病学规律分析的有效方法。To establish the ERIC-PCR（Enterobacterial repetitive intergenic consensus-PCR）DNA fingerprinting technique for typing Haemophilus parasuis and apply it the epidemiological investigation.The result of polymorphism analysis could differentiate the 47 isolates into 28 groups,and the isolates from same farm or of same serotyping showed different genotype.And the isolates wich could not be serotyping could be genotyping by ERIC-PCR.The prevalent isolates gave difference ERIC-PCR fingerprint.ERIC-PCR DNA fingerprinting technique developed in this study is effective way for molecular epidemiological investigation of Hparasuis.

关 键 词：副猪嗜血杆菌 ERIC-PCR 指纹图谱 

分 类 号：S852.61[农业科学—基础兽医学]