45株鸡卡氏杆菌的分离鉴定  

Isolation and preliminary identification of 45 Gallibacterium anatis strains

作　　者：郑鹿平[1] 杨霞[1] 陈陆[1] 皇甫和平[1] 刘慧敏[1] 郭伦涛[1] 徐雪[1] 付仁一[1] 王泽霖[1] 王川庆[1] 

机构地区：[1]河南农业大学禽病研究所,河南郑州450002

出　　处：《中国兽医学报》2010年第3期347-351,共5页Chinese Journal of Veterinary Science

基　　金：中德合作项目(合同号:43006167);该项目由德国柏林格(BoehringerIngelheimVetmedica)公司赞助

摘　　要：通过形态学检查、鉴别培养、Vitek-32全自动细菌鉴定系统、PCR鉴定和序列分析等方法,对101只鸡的10种组织脏器进行细菌分离和鉴定。最终鉴定出45株鸡卡氏杆菌,其中43株分离自输卵管炎产蛋病鸡,2株分离自具有呼吸道病史的产蛋鸡,而从SPF鸡、公鸡及马立克病患鸡体内未能分出卡氏杆菌;分离的卡氏杆菌对庆大霉素、链霉素、氨苄西林、青霉素、强力霉素、诺氟沙星及磺胺类药物等都具有不同程度的耐药性。试验结果表明,卡氏杆菌与产蛋鸡的输卵管炎具有相关性,鸡卡氏杆菌的抗生素耐药现象较普遍,为预防该病原在我国的流行提供了依据。45 Gallibacterium anatis strains from 10 tissues of 101 chickens were isolated and identified by morphological observation,differential cultivation,Vitek-32 automatic bacterial identification system,PCR and sequence analysis.Among the 45 isolates,43 isolates were from hens with salpingitis and 2 isolates were from hens with respiratory symptoms previously.No Gallibacterium anatis was isolated from cocks or SPF hens.All of the 45 Gallibacterium anatis strains had the same drug resistance patterns to cidomycin,phytomycin,ampicillin,laevomycetin,deoxycycline,norfloxacin and sulfonamides.The experimental data indicate clear correlation of Gallibacterium anatis with hen salpingitis and urgent need for further investigation into the drug resistance to effective control of the disease in Chinese poultry flocks.

关 键 词：蛋鸡 输卵管炎 卡氏杆菌 分离鉴定 

分 类 号：S852.61[农业科学—基础兽医学]