抗独特型抗体检测H9亚型禽流感血清抗体  

Initial research on detection of serum antibodies to avian influenza virus subtype H9 by anti-idiotypic antibodies

作　　者：李宝全[1] 彭军[1] 牛钟相[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2010年第3期362-364,共3页Chinese Journal of Veterinary Science

摘　　要：为了探讨用抗独特型抗体替代病毒或病毒亚单位检测H9亚型禽流感血清抗体的可行性,本试验分别用全病毒抗原和抗独特型抗体(Ab2)作检测原,通过琼脂免疫扩散试验(AGP)和间接酶联免疫吸附试验(ELISA)检测了鸡的血清样品。在AGP试验中,分别用Ab2和禽流感病毒(AIV)对10份血清样品检测的符合率为70%。在间接ELISA试验中,Ab2和AIV抗原对10份血清样品检测的符合率为90%。用Ab2间接ELISA试验检测出的血清阳性率(8/10)高于血凝抑制试验(6/10)和AGP试验(5/10)。结果表明,在一定的情况下Ab2可以用来代替具有污染环境风险的病毒抗原来检测抗病毒抗体。To explore the feasibility of detecting serum antibodies to avian influenza virus （AIV） subtype H9 using anti-idiotypic antibodies （Ab2） as a surrogate of virus antigens or virus subunits,AIV antigens and Ab2 was used to detect chicken serum by agar gel precipitation （AGP） and enzyme-linked immunosorbent assay （ELISA）.The result by Ab2 is in accordence with that by AIV antigens at 70%,while at 90% by ELISA.The positive rate by Ab2 ELISA detection （8/10） is higher than that by hemagglutination inhibition test （6/10） and AGP （5/10）.This results indicate in some condition Ab2 can replace AIV antigens which give a risk of pollute environment for detecting antibodies to virus.

关 键 词：禽流感病毒 抗独特型抗体 酶联免疫吸附试验 琼脂免疫扩散试验 

分 类 号：S852.65[农业科学—基础兽医学]