猪干扰素-γ基因多态性及与部分免疫指标关联性分析  

Single nucleotide polymorphism of IFN-γ gene and its association with partial immunity parameters in pigs

作　　者：王大力[1,2] 刘畅[1] 周淑世[1] 岳续朋[1] 刘继丰[1] 戴立胜[1] 孙博兴[1] 赵志辉[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2010年第3期370-374,共5页Chinese Journal of Veterinary Science

基　　金：吉林省科技发展计划资助项目(20080208)

摘　　要：为研究干扰素-γ(Interferon-gamma,IFN-γ)基因作为猪抗病育种中一种分子标记的可能性,采用PCR-SSCP及测序方法对军牧1号白猪、杜洛克猪、约克夏猪3个品种共481头个体IFN-γ基因全序列的单核苷酸多态性进行了检测,结果发现在猪IFN-γ基因的内含子1、内含子3、外显子4内各发现1个突变位点,分别为T825C、T2730C、G5301A。除在约克夏猪中没有检测到G5301A突变外,各遗传群体内T825C、T2730C、G5301A位点均处于Hardy-Weinberg平衡状态。用最小二乘法分析了各个突变位点基因型与部分免疫指标的相关性,结果表明,T825C位点3种基因型间IgG含量AB型显著高于AA型和BB型(0.01<P<0.05);T2730C位点IgM含量CC型显著高于CD型和DD型(0.01<P<0.05);G5301A位点IgG含量EE型显著高于EF型(0.01<P<0.05)。Interferon-gamma（IFN-γ ） plays an important role in the innate host defense against viral infection.In order to analyze the value that the IFN-γ gene act as a genetic marker in porcine disease resistance,single nucleotide polymorphisms （SNPs） in the whole region of the IFN-γ gene were studied by use of PCR-SSCP approach in 481 pigs from the＂Junmu-1＂ White,Landrace,Duroc pigs.Three SNP sites were detected：T825C in intron 1,T2730C in intron 3 and G5301A in extron4.Except for the absence of G5301A in Duroc pigs,all the other sites in the all populations were in a state of Hardy-Weinberg equlibrium.The genetic correlations were obtained between genotypes and several immunity traits by the least square analysis.The results indicated that content of IgG with AA genetype was significantly higher than that of AA and BB on locus T825C（0.01P〈0.05）;the content of IgM with CC genotype was significantly higher than that of CD and DD on locus T2730C（0.01P〈0.05）;the content of IgG with EE genotype was significantly higher than that of EF on locus G5301A（0.01P〈0.05）.

关 键 词：猪 干扰素-γ基因 多态性 免疫指标 关联性 

分 类 号：S852.4[农业科学—基础兽医学]