草原红牛IGFBP3基因多态性及与部分屠宰性状的相关性分析  

Polymorphism of insulin-like growth factor binding protein 3 gene and its associations with several carcass traits in Ckinese red steppe

作　　者：张永宏[1] 高妍[2] 陈承祯[2] 刘同欣[1] 姜昊[1] 戴立胜[1] 孙博兴[1] 张嘉保[2] 赵志辉[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《中国兽医学报》2010年第4期549-551,564,共4页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划项目(2007BAD55B01);国家"863"计划项目(2008AA101010)

摘　　要：以草原红牛为研究对象,采用PCR-SSCP方法检测IGFBP3基因的多态性,并将不同基因型与部分屠宰性状进行相关分析。结果表明在第2内含子发现多态性位点,有AA、AB和BB 3种基因型;测序结果显示在8 069 bp处存在C→T的碱基突变;该位点的多态性对草原红牛的眼肌面积有显著影响(P<0.05),AA型显著高于BB型(P<0.05),但与AB型之间差异不显著(P>0.05);其他屠宰性状在不同基因型间差异不显著。由此可以初步推断IGFBP3基因可能是影响草原红牛肉质性状的一个主效基因或是一个与影响肉质性状的QTL连锁的基因。This study was designed to investigate the single nucleotide polymorphism（SNP） of insulin-like growth factor binding protein 3（IGFBP3） gene in red steppe by PCR-SSCP,and at the same time a correlation between the polymorphisms and several carcass traits.The results of cloning and sequencing indicated that polymorphism of intron 2 was dued to base mutation of C→T in position 8 069,and there were three genotypes（AA,AB and BB）.The correlation analysis revealed that polymorphisms had a significant effect on marbling,the AA genotype had higher marbling than the BB genotype in red steppe（P〈0.05）,but there were no significant differences between AA genotype and AB genotype（P〉0.05）.Other carcass traits of red steppe was not significant in different genotypes（P〉0.05）.It could be preliminarily concluded that IGFBP3 gene was probably a major gene or a QTL linked gene which associated with meat quality traits in red steppe.

关 键 词：草原红牛 胰岛素样生长因子结合蛋白3 PCR-SSCP 屠体性状 

分 类 号：S823.2[农业科学—畜牧学]