病理性瘢痕组织中SPARC的表达变化及意义  

Expression and significance of SPARC in hypertrophic scar and keloid tissue

作　　者：刘超华[1] 李小静[1] 宁金龙[1] 王春[1] 

机构地区：[1]安徽医科大学第一附属医院,合肥230022

出　　处：《山东医药》2010年第14期30-31,共2页Shandong Medical Journal

基　　金：安徽省自然科学基金资助项目(070413102)

摘　　要：目的观察富含半胱氨酸的酸性分泌糖蛋白(SPARC)在瘢痕疙瘩和增生性瘢痕中的表达变化及其意义。方法病理性瘢痕患者52例,其中瘢痕疙瘩(瘢痕组)和增生性瘢痕组织(增生组)各26例,另取正常皮肤组织5例为对照(对照组)。采用免疫组化SP法检测三组SPARC。将三组组织中的成纤维细胞传代培养(4～6代),采用Western blot法检测其SPARC。结果瘢痕组、增生组中SPARC表达较弱,虽比对照组高,但P>0.05;在早期增生性瘢痕(3～6个月)中的表达与晚期(9～12个月)近似(P>0.05)。瘢痕组、增生组成纤维细胞中SPARC表达均高于对照组(P均<0.05)。结论病理性瘢痕组织中的SPARC均呈低表达,其成纤维细胞中的SPARC呈高表达,这可能与病理性瘢痕的发生有关。Objective To investigate the expression and significance of Secreted protein, acidic and rich in cysteine （SPARC） in keloid and hyperplastic scar（HS）. Method Fifty-two patients of pathological scar were applied, which was keloids （ scar group） and HS tissue （HS group） of the 26 cases respectively, and the other 5 cases of normal skin tissue taken as control （control group）. The expression of SPARC in the tissue of 3 groups were detected by immunohistochemical SP method and in the fibroblasts cultured （4 -6 generations） were detected by western blot. Results The expression of SPARC in the tissue between sear group and HS group were stronger than that in the control group, but P 〉 0.05. Early expression of HS was similor to the late HS （ P 〉 0.05 ）. The expression of SPARC in the fibreblasts of sear group and HS group were higher than that of control group （ P 〈 0.05 ）. Conclusions There are low SPARC expression in keloid and HS, and highly expression of fibroblasts in its. The SPARC play a possible role in keleid and HS as fibrosis inducing factor.

关 键 词：瘢痕疙瘩 增生性瘢痕 富含半胱氨酸的酸性分泌糖蛋白 

分 类 号：R619.6[医药卫生—外科学]