氯化血红素催化反应体系的动力学性质及在葡萄糖分析中的应用  

Kinetic Property of the Catalytic Reaction Systen of Haem Chloride and Its Application to Analysis of Glucose

作　　者：高景芝[1] 扬彬[1] 李蓓[1] 李春颖[1] 王孝珍[1] 

机构地区：[1]首都师范大学化学系

出　　处：《分析试验室》1998年第6期35-37,共3页Chinese Journal of Analysis Laboratory

摘　　要：研究了氯化血红素作为过氧化物模拟酶催化显色体系（Ｃ６Ｈ５－ＯＨ－４－ＡＡＰ－Ｈ２Ｏ２）并与葡萄糖氧化反应偶联的动力学性质。通过控制模拟酶和各反应物的用量确定了反应成假一级反应的葡萄糖浓度范围，在此反应体系中，葡萄糖浓度可以通过测定染料的吸光度而获得。该法干扰小，回收率达９４％～９８％。检测限为２．０×１０－４ｇ／Ｌ。In this paper it is reported to investigate kinetic characteristics of Color derveloping system catalyzed by haem chloride as simulation eanzyme of peroxidase and of the coupling reaction of oxidation of glucose,to determine concentration range of glucose in a apparent first order reaction under control of the conentration of the enzyme and some reactants.In this reaction system glucose concentration was determined by absorbance of a dye,and this procedue has low interference satisfactory recovery with 94%～98% and detection limit of 2.0 ×10 -4 g/L of glucose.

关 键 词：氯化血红素 葡萄糖 动力学 测定 模拟酶 人血清 

分 类 号：Q532.303[生物学—生物化学] R446.11[医药卫生—诊断学]