压力流驱动电色谱分析方法及其若干色谱行为  

Theoretical and Experimental Consideration of Chromatographic Behavior in Presurized Flow-Driven Electrochromatography

作　　者：邓玉林[1] 张建华 顾峻岭[1] TakaoTsuda PeterH.Yu 

机构地区：[1]北京理工大学化工与材料学院,北京100081 [2]Universityof Saskatchwan [3]日本名古屋工业大学应用化学系

出　　处：《分析化学》1998年第12期1413-1418,共6页Chinese Journal of Analytical Chemistry

基　　金：北京市自然科学基金!(2952011);教育部外教司基金!(JU98-06)资助课题。

摘　　要：压力流驱动电色谱分析方法是一种新型色谱技术。它组合了ＨＰＬＣ和电泳两种技术，为分离提供了更多可控制的因素。介绍了压力流驱动电色谱的装置和毛细管电色谱柱的装填方法，从理论上讨论了影响电色谱分离的因素并用实验数据加以证明。最后尝试性地将电色谱法用于测定香蕉样品中萨索啉（Ｓａｌｓｏｌｉｎｏｌ）等的含量。A novel chromatographic technique, pressurized flow-driven electrochromatography, that combines the features of both capillary high parformance liquid chromatography and capillary electiophoresis is introduced, in this paper. We report the investigation of the chromatographic behavior using a home-constructed electrochromatographic system coupled with an ODS-packed capillary column. Experimental results were discussed in relation to theoretical considerations. We are also interested in exploring the potential advantages offered by pressurized flow-driven electrochromatography, e. g, oncolumn sample focusing and high resolution ability. fo our preliminary study, the determination of salsolinol level in banana was used as an example of application of this technique.

关 键 词：电角谱 电渗流 分离 压力流驱动 毛细管电色谱 

分 类 号：O657.7[理学—分析化学]