高效液相色谱法测定皮炎宁酊中间苯二酚和水杨酸的含量  

Determination of Resorcinol and Salicylic Acid in Piyanning Tincture by High Performance Liquid Chromatography

作　　者：郭兴杰[1] 周密[1] 

机构地区：[1]沈阳药科大学分析教研室

出　　处：《色谱》1998年第6期532-533,共2页Chinese Journal of Chromatography

摘　　要：采用高效液相色谱法测定了皮炎宁酊中间苯二酚和水杨酸的含量。色谱柱为ＨｙｐｅｒｓｉｌＯＤＳ柱，流动相为Ｖ（甲醇）∶Ｖ（水）∶Ｖ（乙酸）＝５０∶５０∶０．９。检测波长为２８５ｎｍ。在此条件下，间苯二酚和水杨酸可与其降解产物及杂质完全分开。A method for the simultaneous determination of resorcinol and salicylic acid in Piyanning tincture by HPLC has been proposed. Operating conditions were Hyppersil ODS column, 4 6 mm×200 mm, V (methanol)∶ V (water)∶ V (acetic acid)=50∶50∶0 9 mobile phase and UV detection at 285 nm. The linear ranges of the method were 0 05 0 25 g/L( r =1 000) for resorcionl and 0 025 0 127 g/L( r =1 000) for salicylic acid. The limits of detection were both 0 2 mg/L at a signal to noise of 3 The assay method was capable to resolve resorcinol and salicylic acid from their impurities.

关 键 词：皮炎宁酊剂 间苯二酚 水杨酸 HPLC 分析 

分 类 号：R986[医药卫生—药品]