可卡因对小鼠脾细胞内ATP酶、LDH和SDH活性的影响  

Effects of Cocaine on Activities of ATPase,LDH and SDH in Mouse Splenocytes

作　　者：孙文平[1] 卢延旭[2] 张晓宇 唐玮玮[2] 黄庆玉[1] 

机构地区：[1]泰山医学院病理教研室,山东泰安271000 [2]中国刑警学院法医病理教研室,辽宁沈阳110035 [3]内蒙古自治区国家安全厅,内蒙古呼和浩特010010

出　　处：《法医学杂志》2010年第2期81-83,共3页Journal of Forensic Medicine

基　　金：国家"十五"科技攻关项目(2001BA801B04)

摘　　要：目的考察盐酸可卡因对体外培养小鼠脾细胞内ATP酶、乳酸脱氢酶(lactate dehydrogenase,LDH)和琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性的影响。方法采用小鼠脾细胞体外培养的方法,在脾细胞培养液中加入终质量浓度分别为10、20和100μg/mL的盐酸可卡因,按试剂盒所述条件在7d时检测脾细胞中ATP酶、LDH和SDH活性。结果在离体脾细胞培养液中加入不同剂量的可卡因,培养7d,脾细胞内ATP酶、LDH与SDH活性均明显降低。结论可卡因对脾细胞内ATP酶、LDH和SDH活性有抑制作用。Objective To examine the effects of cocaine on the activities of ATPase, LDH and SDH in cultured mouse splenocytes in vitro. Methods The ATPase, LDH and SDH activities in mouse splenocytes were detected at day 7 after continuous culturing the mouse cells exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 μg/mL in vitro. Results The activities of ATPase, LDH and SDH in mouse splenocytes exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 μg/mL were significantly decreased after continuous cuhuring for 7 days. Conclusion The present study demonstrated that cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes in vitro.

关 键 词：法医病理学 可卡因 脾 腺苷三磷酸 乳酸脱氢酶 琥珀酸脱氢酶 小鼠 

分 类 号：R992[医药卫生—毒理学]