UVA辐射对HaCaT细胞分泌和表达趋化因子CXCL11／I—TAC的影响  

Influences of ultraviolet A （UVA） on the secretion and expression of chemokine CXCLll/I-TAC by HaCaT cells

作　　者：耿士玲[1] 单士军[1] 张同威[1] 吴剑[1] 王志华[1] 肖汀[1] 何春涤[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科,沈阳110001

出　　处：《中华皮肤科杂志》2010年第3期181-183,共3页Chinese Journal of Dermatology

基　　金：国家自狐科学基金（30400389）;教育部高等学校博士学科点专项科研基金（20060159014）,教育部创新团队基金（IRT0760）,辽宁省创新团队基金（2008T192）

摘　　要：目的探讨UVA对IFN-γ和TNF—α诱导的HaCaT细胞分泌和表达CXCL11／I—TAC的影响。方法用ELISA检测不同剂量UVA（2、4、8J／cm^2）照射后培养24h的HaCaT细胞上清中CXCL11／I-TAC分泌水平。用实时荧光定量PCR检测CXCLl1／I—TACmRNA表达水平。结果正常培养的HaCaT细胞仅分泌和表达微量的CXCL11／I—TAC蛋白或mRNA。当用10μg/L的IFN-γ和TNF—α联合刺激后，HaCaT细胞分泌或表达的CXCLI1／I-TAC显著升高。UVA在2、4、8J／cm^2呈剂量依赖性抑制CXCL11／I—TAC的分泌或表达。结论UVA照射抑制角质形成细胞分泌和表达CXCL11／I—TAC，从而在一定程度上降低了对Th1／Te1细胞的趋化。Objective To investigate the influences of UVA on the secretion and expression of chemokine CXCLll/I-TAC by HaCaT cells induced by interferon γ （IFN-γ） and tumor necrosis factor α （TNF-α）. Methods HaCaT ceils were cultured in the presence of IFN-γ and TNF-α and irradiated with UVA of 2, 4 and 8 J/cm^2, respectively; those cells receiving neither treatment with IFN-γ or TNF-α nor UVA irradiation served as the negative control, and those receiving only cytokine treatment but no irradiation as the positive control. After another 24-hour culture, enzyme-linked immunosorbent assay （ELISA） was performed to detect the protein levels of CXCL11/I-TAC in the supernatant of HaCaT cells, real time PCR to measure the mRNA expression of CXCL11/I-TAC in these HaCaT cells. Results As far as the negative control HaCaT cells were concerned, there was a minor secretion of CXCL11/I-TAC protein and expression of CXCL1 I/I-TAC mRNA. After treatment with IFN-γ and TNF-α of 10 μg/L, the protein and mRNA expressions of CXCLI1/ I-TAC were synergistically upregulated, whereas the induced secretion and expression of CXCL11/I-TAC by HaCaT cells were dose-dependently inhibited by UVA irradiation. Conclusions UVA irradiation inhibits the secretion and expression of CXCL11/I-TAC by HaCaT cells, which in turn suppresses the chemotaxis of Th1/ Tc 1 ceils in some degree.

关 键 词：紫外线 HACAT细胞 趋化因子 CXC 

分 类 号：R751[医药卫生—皮肤病学与性病学]