尼罗罗非鱼微卫星标记与主要生长性状的相关性分析  

Correlation analysis of microsatellite DNA markers with major growth traits of tilapia(Oreochromis niloticus)

作　　者：刘福平[1,2] 白俊杰[1] 宋红梅[1] 叶星[1] 李胜杰[1] 于凌云[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《水产学报》2010年第2期169-177,共9页Journal of Fisheries of China

基　　金：农业部"948"项目(2006-G55);国家"十一五"科技支撑计划(2006BAD01A1201);国家科技基础条件平台工作项目(2005DKA21103)

摘　　要：为了筛选到与尼罗罗非鱼生长相关的分子标记,并对这些标记进行准确性鉴定,运用65个微卫星标记对鹭业和番禺2个尼罗罗非鱼群体进行了PCR扩增,再利用SPSS软件一般线性模型(GLM)对这些微卫星位点与尼罗罗非鱼体重等主要生长性状进行了标记性状连锁关联分析。结果表明,鹭业群体中有8个微卫星标记(UNH130,UNH183,UNH911,GM558,UNH211,UNH176,UNH914和UNH974)与主要生长性状显著或极显著相关(P<0.05或P<0.01),其中有2个微卫星标记(UNH914和UNH974)与番禺群体的主要生长性状显著或极显著相关(P<0.05或P<0.01),1个微卫星标记(UNH176)只与体重显著相关(P<0.05)。通过对与生长性状相关标记的基因型和表型值进行多重比较,得到了对体重、体长和体高3种性状有利的基因型或等位基因。发现了对罗非鱼生长性状有显著效应的微卫星位点,为开展罗非鱼的分子标记辅助育种提供了有价值的遗传标记。In order to obtain more molecule markers linked to growth-related traits of tilapia and evaluate their accuracy, 65 microsatellites loci were amplified in two Nile tilapia populations （Luye and Panyu）, and the marker-trait association was analyzed by GLM（ general linear model）procedure of SPSS software. The correlation analysis results showed that 8 loci （UNH130, UNH183, UNH911, GM558, UNH211, UNH176, UNH914 and UNH974 ） had a significant impact on major growth traits （weight, body length and body height） of Luye population （P 〈 0.05 or P 〈 0.01 ）. In Panyu tilapia population, UNH914 and UNH974 had a significant impact on major growth traits （P 〈0.05 or P 〈0.01 ）, and UNH176 just had a significant impact on body weight （P 〈 0.05 ）. The favorable genotypes or allele linked to growth traits including weight, body length and body height were determined by multiple comparison analysis between the phenotypic value and different genotypes of microsatellites markers linked to major growth traits. In this study, the microsatellite loci with a significant effect on growth traits were found, and they were potential valuable genetic markers for marker-assisted selection of tilapia.

关 键 词：尼罗罗非鱼 微卫星标记 生长性状 相关性分析 

分 类 号：Q953[生物学—动物学] S917[农业科学—水产科学]