胸腺基质细胞对热应激小鼠胸腺细胞亚群变化及HSP70表达的影响  

Effects of thymic stromal cells on subpopulation and HSP70 expression of heat stress-treated thymocytes in vitro

作　　者：蒋定文[1] 郭明秋[2] 陈立茵[2] 沈先荣[1] 陈伟[1] 何颖[1] 

机构地区：[1]海军医学研究所防护医学研究室,上海200433 [2]南方医科大学基础部抗衰老研究室,广州510515

出　　处：《免疫学杂志》2010年第3期191-196,共6页Immunological Journal

摘　　要：目的探讨胸腺基质细胞(TSC)对热应激小鼠胸腺细胞的调节作用。方法应用光镜、电镜、流式细胞术等观察初代培养的TSC对热应激胸腺细胞发育的调节作用。结果 TSC可大量粘附和吞噬胸腺细胞,使热应激胸腺细胞数量明显减少,但尚存的胸腺细胞中活细胞比例却明显增加,凋亡率明显减少。TSC可促进热应激胸腺细胞HSP70表达增加,使热应激胸腺细胞的DP及SP细胞,尤其是DP细胞数量明显减少。结论 TSC可清除大量的热应激胸腺细胞,TSC促进热应激胸腺细胞HSP表达增高可能具有双重意义:既可保护胸腺细胞,又可促进TSC识别和吞噬已受损的或凋亡的胸腺细胞。We aimed to explore the regulatory effects of primary cultured thymic stromal cells （TSC） on heat stress-treated thymocytes. Light microscopy, electron microscopy, and flow cytometry （FCM） were performed to observe the regulation effect of primary cultured TSC on heat stress-treated thymocytes in vitro. Heat stress-treated thymoeytes were adhered and swallowed by TSC, which caused significant decrease of thymocytes, but the remained thymocytes displayed high survival rate and low apoptotic rate. When thymocytes were cocultured with TSC, the positive ratio of HSP70 expression in heat stress-treated thymocytes increased obviously, while both DP and SP, especially immature DP thymocytes, decreased significantly. The results mean that the increased expression of HSP70 on heat stress-treated thymocytes cocultured with TSCs may exert dual functions： One is protecting of thymocytes from apoptosis; the other is recognizing and engulfing damaged or apoptotic thymocytes.

关 键 词：胸腺基质细胞 胸腺细胞 热应激 CD4CD8细胞亚群 热休克蛋白70 

分 类 号：R392.12[医药卫生—免疫学]