氟达拉滨对多发性骨髓瘤细胞系KM3细胞增殖和代谢的影响  

Effects of fludarabine on the autocrining cytokines in multiple myeloma KM3 cells

作　　者：周亚丽[1] 彭志刚[2] 张晓梅[2] 罗军[2] 赖永榕[2] 

机构地区：[1]解放军303医院血液科,南宁530021 [2]广西医科大学第一附属医院血液科

出　　处：《临床血液学杂志》2010年第2期104-106,共3页Journal of Clinical Hematology

摘　　要：目的:研究氟达拉滨在体外对多发性骨髓瘤细胞系KM3细胞的生长抑制、诱导凋亡作用及对细胞自分泌细胞因子的影响。方法:采用MTT法观察不同浓度氟达拉滨对KM3细胞生长的影响;用AnnexinⅤ/PI双染法流式细胞仪检测KM3的凋亡率;用双抗夹心法(ELISA)检测上清液中VEGF、IL-6和可溶性IL-6受体(sIL-6R)的水平。结果:①氟达拉滨对KM3细胞有明显的抑制作用,并随药物浓度升高和作用时间的延长而抑制作用增强。MTT法显示,氟达拉滨作用于KM3细胞72h后,其半数抑制率IC50约为95nmol/L。25～400nmol/L浓度范围氟达拉滨作用72h后,KM3细胞的凋亡率随药物浓度的增加而升高。②KM3细胞经氟达拉滨作用72h后,上清液中VEGF和sIL-6R水平随药物浓度的升高逐渐下降,而IL-6水平先升高后明显下降。结论:氟达拉滨能明显抑制KM3细胞的增殖并诱导其细胞凋亡,同时能抑制KM3细胞自分泌VEGF、IL-6和sIL-6R。Objective：To study the effects of fludarabine on growth of multiple myeloma KM3 cells and the influence of fludarabine on autocrining level of cytokines.Method：The effects of different concentrations of fludarabine on the proliferation of KM3 multiple myeloma cells were examined by MTT assay. The apoptosis of KM3 cells was stained with AnnexinⅤ/PI and detected by flow cytometr. The levels of IL-6 and sIL-6R autosecreted by KM3 cells were tested by ELISA after KM3 cells were treated with fludarabine. Result：Fludarabine inhibited the proliferation of KM3 cells and the inhibition effect were dependent on time and concentration.The MTT assay showed that the IC50 was 95 nmol/L with fludarabine treatment for 72 hours. The apoptosis rate of KM3 cells increased with fludarabine treatment at a dosage within 25400 nmol/L. Fludarabine decreased the autocrining level of IL-6 slightly,and inhibited the level of VEGF and sIL-6R obviously. Conclusion：The proliferation of KM3 cells was markedly inhibited by fludarabine in a dose-dependent and time-dependent manner. Fludarabine can also decrease the level of VEGF,IL-6 and sIL-6R autocrined by KM3 cells.

关 键 词：氟达拉滨 KM3系细胞株 生长抑制 细胞因子 

分 类 号：R979[医药卫生—药品]