国产与进口胎牛血清培养MDCK细胞及分离流感病毒的效果比较  

Effectiveness Comparison of Domestic and Imported Fetal Bovine Serums in Cultivation of MDCK Cells and Isolation of Influenza Virus

作　　者：龙歆孜 石文松[1] 

机构地区：[1]湖南省株洲市疾病预防控制中心,湖南株洲412000

出　　处：《实用预防医学》2010年第2期370-371,共2页Practical Preventive Medicine

摘　　要：目的比较某品牌国产与进口胎牛血清用于培养MDCK细胞及分离流感病毒的效果。方法除胎牛血清外,在其它条件相同的情况下培养同批MDCK细胞,比较贴壁情况和细胞形态。选择在两种培养条件下形态良好的细胞,接种流感阳性标本,比较二者的阳性分离率差异及病毒效价。结果在细胞贴壁情况、形态(χ2=22.76,P<0.05)及标本阳性分离率、病毒效价方面(χ2=7.52,P<0.05),进口胎牛血清优于国产试剂,试验结果差异有统计学意义。结论为提高流感监测的阳性分离率,提倡使用进口试剂。Objective To compare the effectiveness between a certain domestic brand of fetal bovine serum and an imported one on culturing MDCK cells and isolating influenza viruses.Methods The same batch MDCK cells were cultured under the same conditions except domestic or imported fetal bovine serum,and then the adherent rate and morphology of the cells in the two groups were compared.The cells with good morphology of the two groups were inoculated with the influenza-positive specimens,and then the positive rate and virus titers were compared between the two groups.Results The difference of adherent rate and morphology between the cells in the two groups was statistically significant （χ2=22.76,P0.05） and so was the isolation rate of positive specimens and virus titers （χ2=7.52,P0.05）.The imported fetal bovine serum was superior to the domestic one.Conclusions To improve the positive isolation rate of influenza virus,it is suggested to use the imported fetal bovine serum.

关 键 词：胎牛血清 MDCK细胞 流感病毒 

分 类 号：R373.13[医药卫生—病原生物学]