谷氨酸脱羧酶活性测定研究  

Study on Determination of The Activity of L-Glutamic Decarboxylase

作　　者：吴国琪[1] 凌达仁[1] 黄昕 

机构地区：[1]江苏石油化工学院应用化学系

出　　处：《江苏石油化工学院学报》1998年第3期43-46,共4页Journal of Jiangsu Institute of Petrochemical Technology

基　　金：江苏省科委资助项目

摘　　要：酶活性测定是进行酶研究的关键，运用ＭＩＡ－３型微机化多功能离子分析仪，与ＣＯ２气敏电极相匹配，确定了谷氨酸脱羧酶活性测定体系。研究了不同的缓冲体系对酶活性测定的影响，确定了ｐＨ为４４，０１０ｍｏｌ／ＬＮａ２ＨＰＯ４－００５ｍｏｌ／Ｌ柠檬酸缓冲溶液作为反应缓冲体系。测定了谷氨酸脱羧酶的最适ｐＨ（５０）和最适温度（５５℃），确定了ＧＤＣ活性测定体系为：ｐＨ为４４，０１０ｍｏｌ／ＬＮａ２ＨＰＯ４－００５ｍｏｌ／Ｌ柠檬酸缓冲溶液，反应温度为３７℃，溶液中底物浓度为１２ｍｍｏｌ／Ｌ。经测定谷氨酸脱羧酶活性为１７６ｍｍｏｌ／Ｌ·ｍｉｎ，相对平均偏差小于１％。The determination of the activity of enzyme is the key to its study.The activity of L-Glutamic decarboxylase is determined by the MIA-3 micro computerized multifunctional ion analyzer coupled with CO 2 electrode. The effect of buffer solution system on the determination of the enzymatic activity is studied,the optimum pH of the L-glutamic decarboxylase is 5 0,its optimum temperature is 55℃.The analysis system of the enzymatic activity is determined:The buffer solution is pH=4 4 0 10mol/L Na 2HPO 4～0 05mol/L citric acid,the reactive temperature is kept in (37±0 2)℃ for some reasons,the substrate concentration in solution is 12mmol/L.The experiment shows that the activity of L-glutamic decarboxylase equals 1 76mmol/L·min, the relative average error less than 1%.

关 键 词：谷氨酸脱羧酶 谷氨酸 二氧化碳电极 酶活性 

分 类 号：O657.5[理学—分析化学] Q550.3[理学—化学]