生态养猪发酵床菌种产蛋白酶特性的研究  

Characteristics of Protease Produced by Microbes in Fermentation Bed for Ecological Pig-raising

作　　者：尹红梅[1] 张国民[2] 郭照辉[1] 谭周进[3] 贺月林[1] 

机构地区：[1]湖南省微生物研究所,湖南长沙410005 [2]湖南农业大学,湖南长沙410128 [3]湖南中医药大学,湖南长沙410208

出　　处：《湖南农业科学》2010年第2期116-118,共3页Hunan Agricultural Sciences

基　　金：湖南省科技厅重大专项(2009FJ1005)

摘　　要：采用福林法对武汉芽孢杆菌、枯草芽孢杆菌、7号芽孢杆菌、9号芽孢杆菌、Y2芽孢杆菌、BB1芽孢杆菌进行发酵后产蛋白酶活力测定。结果表明:7号芽孢杆菌、9号芽孢杆菌、Y2芽孢杆菌产蛋白酶活力较小,武汉芽孢杆菌、枯草芽孢杆菌、BB1芽孢杆菌产蛋白酶活力较高;在三株产蛋白酶活力高的菌种培养基中加入微量元素,实现产酶条件优化,结果是:对武汉芽孢杆菌产蛋白酶有较大影响,对枯草芽孢杆菌和BB1芽孢杆菌影响较小。The activities of proteases that produced by fermented Bacillus Wuhan, Bacillus subtilis, Bacillus 7^th, Bacillus 9^th, Bacillus Y2 and BB1 Bacillus were determined by Folin method. The results showed that proteases produced by Bacillus 7^th, Bacillus 9^th and Bacillus Y2 had lower activities, hut proteases produced by Bacillus Wuhan, Bacillus subtilis and BB1 Bacillus had much higher activities. Then, add trace elements to the three strains＇ mediums that with high producing protease activity to optimize conditions of enzyme production. The results indicated that it had a large impact on the protease production of Bacillus Wuhan, but it had a small impact on the protease production of Bacillus subtilis and BB1 Bacillus.

关 键 词：发酵床养猪 芽孢杆菌 福林法 蛋白酶 酶活力 

分 类 号：S816.7[农业科学—饲料科学]