内脏神经-体神经端侧吻合后神经再生方式的研究  

A retrograde tracer study of the regenerative nerve after end-to-side nerve anastomosis of distal stump of autonomic nerve to somatic nerve in rats

作　　者：李兵[1] 高宛生[1] 董传江[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022

出　　处：《中华实验外科杂志》2010年第3期336-337,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30500535、30872567）

摘　　要：目的通过内脏神经-体神经端侧吻合建立人工体神经-内脏神经反射弧大鼠模型25只，荧光双标追踪技术研究神经再生方式。方法在成活的21只模型鼠左侧盆神经节（MPG）内注射0．5μl荧光金（FG），腰4前根（L4VR）的外周神经-坐骨神经注射1μl快蓝（FB）进行逆行神经追踪。应用组织化学方法对吻合口远端的神经根（kVR）进行甲苯胺蓝染色。结果逆行追踪结果显示FG单标和FG—FB双标神经元主要分布于脊髓L4左侧前角，FB单标神经元主要分布于脊髓L3-L6左侧前角。脊髓腰4节段每张切片上FG单标神经元、FB单标神经元、FG—FB双标神经元数目分别为（20．8±3．3）、（5．7±1．3）、（10．3±0．7）。吻合口远端（L6VR）再生的神经经甲苯胺蓝染色后可见轴突呈深蓝色。结论逆行追踪结果证明内脏神经-体神经端侧吻合建立人工体神经-内脏神经反射弧后，体神经可以通过侧枝发芽的方式长入并替代内脏神经。Objective To evaluate the collateral sprouting after end-to-side nerve anastomosis of distal stump of autonomic nerve to somatic nerve using a fluorescent double-labeling technique in rats. Methods In group Ⅰ（ end-to-side anastomosis）, 25 Sprague-Dawley （SD） rats had the left lumbar 6 ventral root transected in dura and the distal stump was sutured to the lateral face of left lumbar 4 ventral root without removing the epineurium. After 16 weeks, 0.5 μl Fluorogold （FG） and 1μl Fast Blue （FB） were respectively injected into the left major pelvic ganglia （MPG） and peripheral sciatic nerve （SN） for fluorescent retrograde tracing in 21 survival rats. The regenerative nerve was stained with toluidine blue through the use of histochemical technique. In groop Ⅱ（ control）, five SD rats have not been done any surgery and were treated the same fluorescent double-labeling as model rats of group Ⅰ. Results In group Ⅰ, after FG was injected into the left MPG and FB was injected into SN, FG single labeled neurons and FG-FB dual labeled neurons were observed in locus of the left ventral horn of lumbar 3 caudal to lumbar 5 rostral, mainly in the left ventral horn of lumbar 4, FB single labeled neurons in locus of the left ventral horn of lumbar 3 caudal to lumbar 6. The number of FG single labeled neurons, FB single labeled neurons and FG-FB dual labeled neurons were （ 20.8± 3.3 ）, （ 5.7 ±1.3 ）, （ 10.3 ±0. 7 ） in every section of lumber fourth ventral horn, respectively. The recipient nerve axons were dyed blue after toluidine blue labeling. In control group, FG single labeled neurons were observed in locus of sacral parasympathetic nucleus of lumbar 6 to sacral 1 segment, FB single labeled neurons in locus of the left ventral horn of lumbar 3 caudal to lumbar 6, and FG-FB dual labeled neurons were not found. Conclusion The somatic motor axon ingrowth into autonomic nerve could be through collateral sprouting after end-to-side nerve anastomosis of distal stump of autonomic nerv

关 键 词：端侧吻合 神经再生 体神经 内脏神经 

分 类 号：R651.3[医药卫生—外科学]