祛湿化瘀方改善脂肪肝大鼠游离脂肪酸代谢的机制  

Mechanism of Qushi Huayu Decoction in Improving Metabolism of Free Fatty Acid in Fatty Liver Rats

作　　者：李红山 冯琴[1,2] 胡义扬[1,2] 彭景华[1,2] 顾宏图[1,2] 许丽莉[1,2] 刘成[1,2] 

机构地区：[1]上海中医药大学附属曙光医院 [2]上海市高校中医内科学E-研究院 [3]宁波市传染病医院

出　　处：《中医杂志》2010年第3期262-264,共3页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672635);上海市优秀学科带头人计划资助项目(06XD14018);上海高校创新团队建设项目

摘　　要：目的从"脂联素-游离脂肪酸代谢"路径,探讨祛湿化瘀方对实验性脂肪肝游离脂肪酸抑制作用的机理。方法运用单纯高脂饮食诱导大鼠脂肪肝模型,自造模第7周起,21只大鼠被随机分为模型组和祛湿化瘀方高、低剂量组,每组7只,灌胃饮用水或给药4周。观察肝组织游离脂肪酸(FFA)、甘油三酯(TG)、肝脂肪变性程度、血清脂联素(ADP)、肝组织脂联素受体(AdipoR2)、腺苷酸活化的蛋白激酶(AMPK)、脂肪酸合成酶(FAS)、乙酰辅酶A羧化酶(ACCase)、丙二酰辅酶A(Malonyl-CoA)含量的变化,并与正常组进行对照。结果与模型组比较,祛湿化瘀方高、低剂量组的肝组织TG、FFA含量、FAS、ACCase和Malonyl-CoA显著降低(P<0.01或P<0.05),而AdipoR2、AMPK、血清ADP含量显著升高,并呈量效关系(P<0.05或P<0.01)。结论祛湿化瘀方对"脂联素-游离脂肪酸代谢"路径有显著的干预效应,其对脂联素及其受体的作用是该方降低肝组织FFA从而减轻肝脏脂质沉积的重要机理之一。Objective To investigate the mechanism of Qushi Huayu Decoction （Decoction for dissolving damp and removing stasis） in inhibiting the experimental free fatty acid expression based on ADP-FFA pathway. Methods SD male rats were used to es- tablish fatty liver models by high-fat diet. At the beginning of the seventh week of modeling, 21 rats were randomized into the model group, Qushi Huayu Decoction high-dose and low-dose groups, with 7 in each. They were given water or the deeoetion of different dosage by gavage for 4 weeks. Changes in triglyeeride （TG） and Free Fatty Aeid （FFA） in liver tissue, hepatic steatosis, serum adiponeetin （ADP）, adiponeetin receptor 2 （Adipo R2）, Adenosine Monophosphate-aetivated Protein Kinase （AMPK）, fatty acid synthase （FAS）, Aeetyl-Coenzyme A Carboxylase （ACCase）, and malonyl eoenzyme A （Malonyl-CoA） in liver tissue were observed, and were compared with those of the normal group. Results Compared with the model group, TG and FFA, FAS, ACCase, and Malonyl-CoA in liver tissue of Qushi Huayu Decoction high-dose and low-dose groups were redueed significantly （P^0. 01 or P^0. 05）, while the Adipo R2 and AMPK in liver tissue and ADP in serum were increased markedly, showing a dose-effect relationship （P d0. 05 or P〈~0. 01）. Conclusion Qushi Huayu Decoction has a good effect on the FFA metabolism through the ADP-FFA path- way. Improving serum ADP and hepatic Adipo R2 levels significantly is one of its important mechanisms in inhibiting hepatic FFA so as to reduce hepatic lipid deposited.

关 键 词：祛湿化瘀方 脂肪肝 脂联素 腺苷酸活化的蛋白激酶 游离脂肪酸 

分 类 号：R285.5[医药卫生—中药学]