蒲薏颗粒的定性定量研究  

Study on qualitative and quantitative methods of puyi granules

作　　者：李勇军[1] 何迅[1] 郑林[1] 王爱民[1] 王永林[1] 兰燕宇[1] 

机构地区：[1]贵阳医学院药学院,贵阳550004

出　　处：《中国实验方剂学杂志》2010年第4期24-27,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2006BAI06A01-03);贵州省科技计划项目(黔科合计工字[2009]4001)

摘　　要：目的:建立蒲薏颗粒的定性定量方法。方法:采用薄层色谱法对蒲薏颗粒中的何首乌、蒲公英、薏苡仁和灵芝进行定性鉴别;采用HPLC法对其何首乌中的大黄素进行含量测定。结果:在薄层色谱中何首乌、蒲公英、薏苡仁和灵芝能得到很好的鉴别;大黄素在0.073～1.171μg范围内线性关系良好,平均回收率为99.0%,RSD为2.2%(n=9)。结论:该方法准确、简便、重复性好,可用于可用于蒲薏颗粒的质量控制。Objective：To establish the qualitative and quantitative methods for Puyi Granules.Method： Radix Polygoni Eultiflori,Herba Taraxaci,Semen Coicis and Ganoderma were identified by TLC,and the content of emodin in the preparation was determined by HPLC.Result： Radix Polygoni Eultiflori,Herba Taraxaci,Semen Coicis and Ganoderma could be identified well by TLC.The regression curve of emodin showed a good linear rela-tionship in the range of 0.073 ～ 1.171 μg.The average recovery was 99.0%,RSD was 2.2%.Conclusion： The method is accurate,simple and has good repeatability.It can be used for quality control of Puyi Granules.

关 键 词：蒲薏颗粒 薄层色谱法 高效液相色谱法 大黄素 

分 类 号：R284.1[医药卫生—中药学]