中药胃康宁防治胃癌作用的机制探讨  

Effects and mechanisms of Weikangning on gastric cancer

作　　者：钟娃[1] 阚方巨[2] 于钟[1] 闵存云[2] 李庆明[1] 

机构地区：[1]广州中山大学附属第二医院消化科,广州510120 [2]广州中山大学附属第二医院中医科,广州510120

出　　处：《中国实验方剂学杂志》2010年第4期109-113,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学项目(7001589);广东省中医药管理局课题(1060168)

摘　　要：目的:观察健脾解毒散结中药胃康宁含药血清对胃癌细胞周期调控因子CyclinD1,CyclinD2,Cdk4(Cyclin dependant kinase4,CDK4),Cdk6和p16INK4amRNA及其蛋白表达的作用。方法:分别将高、中、低不同剂量的中药胃康宁制剂igSD大鼠,制备含药血清,然后分别用不同剂量含药血清培养胃癌细胞;采用免疫组织化学SABC法及RT-PCR法检测各组胃癌细胞中CyclinD1,CyclinD2,Cdk4,Cdk6和p16INK4amRNA和蛋白的表达。结果:免疫组化结果显示,与空白对照组比较,胃康宁高、中、低剂量组CyclinD1灰度值变化,无统计学意义(P>0.05);CyclinD2,Cdk4,Cdk6灰度值均有明显升高,而p16INK4a灰度值则显著降低(P<0.05,P<0.01);RT-PCR结果显示,胃康宁高、中、低剂量组CyclinD1 OPTDI值变化,无统计学意义(P>0.05);但能明显降低CyclinD2,Cdk4和Cdk6 OPTDI值,同时升高p16INK4a OPTDI值(P<0.05,P<0.01)。结论:中药胃康宁可抑制胃癌细胞促细胞周期相关因子CyclinD2,Cdk4和Cdk6的表达,同时增强胃癌细胞周期抑制因子p16INK4a的表达,这可能是健脾解毒散结中药胃康宁抑制胃癌细胞生长的作用机制。Objective：To study the effects and mechanisms of Chinese herbal medicine—Weikangning on gastric cancer.Method：Total of 120 SD rats were divided into control group,high dose group,medium dose group and low dose group,and fed with natural saline,20,10 and 5 g /kg of Weikangning decoction respectively.The experimental animals were finally killed for the preparation of drug-containing serum.The gastric cancer cell MGC-803 was cultured with the drug-containing serum drawn from the rats in different groups.The expressions of CyclinD1,CyclinD2,Cdk4,Cdk6 and p16INK4a were detected with immunohistochemistry method-SABC.The expression of mRNA of CyclinD1,CyclinD2,Cdk4,Cdk6 and p16INK4a were detected with RT-PCR.Result：In high,medium and low dose group,the gray scales and the OPDTI value of CyclinD1 had no remarkable change;the gray scales of CyclinD2,Cdk4,Cdk6 and the OPDTI values of p16INK4a increased significantly,but the gray scales of p16INK4a and the OPDTI values of CyclinD2,Cdk4,Cdk6 decreased remarkably,compared with those in control groups.Conclusion： The Chinese herbal medicine Weikangning decoction can decrease the expressions of CyclinD2,Cdk4,Cdk6,increase the expression of p16INK4a.This effect may be involved in mechanism of gastric cancer cell growth inhibition gain by the Weikangning decoction.

关 键 词：中药胃康宁 胃癌 机制 

分 类 号：R285.5[医药卫生—中药学]