葛脾煎剂调控单核细胞趋化蛋白-1水平逆转早期糖尿病肾病的实验研究  

Study of Ge Pi Decoction on regulation of monocyte chemoattractant protein-1 level to reverse early diabetic nephropathy

作　　者：姚民秀[1] 徐倩[1] 商永芳[1] 李盈[1] 王会玲[1] 张方华[1] 

机构地区：[1]青岛大学医学院第二附属医院内分泌科,山东青岛266042

出　　处：《中国实验方剂学杂志》2010年第4期145-148,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨葛脾煎剂对单核细胞趋化蛋白-1(MCP-1)的影响及其对逆转早期糖尿病肾病(DN)的作用。方法:选择Wistar雄性大鼠,采用高脂、高糖饮食联合小剂量链脲佐菌素(STZ)腹腔注射诱导的STZ糖尿病大鼠共41只,随机分为STZ糖尿病大鼠对照组14只,中药(葛脾煎剂)治疗组13只,西药(苯那普利)治疗组14只,分别观察8周;检测各组体重、肾重指数、血糖、尿微量白蛋白、血尿MCP-1、血8-异前列腺素F2α(8-iso-PGF2α)、肿瘤坏死因子-α(TNF-α),并进行肾脏病理学检查。结果:中药治疗组肾脏重量和肾重指数低于对照组(P<0.05),UAER、血尿MCP-1、血8-iso-PGF2α和TNF-α均明显低于对照组(P<0.01);而两治疗组,对上述指标的影响基本类似,两组间无明显差异(P>0.05)。另外,各组8-iso-PGF2α、MCP-1、TNF-α均与UAER呈正相关关系。中药葛脾煎剂能减轻肾脏肥大及肾小球系膜基质增生、硬化。结论:糖尿病肾病有自身免疫性炎症和氧化应激反应参入,中药葛脾煎剂对多种炎症因子具有抑制作用,从而可逆转早期糖尿病肾病。Objective：To investigate the effect of Ge Pi decoction on regulation of monocyte chemoattractant protein-1（MCP-1）level to reverse early diabetic nephropathy（DN）.Method： STZ diabetes model were induced by high lipoids and high glucose diet combined with intraperitoneal injection of small dose streptozotocin（STZ）（n = 41）.STZ-induced diabetes model of Wistar male rats were divided into the control group（n = 14）,the Traditional Chinese medicine（Ge Pi Decoction） treatment group（n = 13）,and the western medicine（benazepril） treatment group（n = 14）.After 8 weeks,detection of each group were carried out about weight,kidney weight index,blood glucose,urinary albumin microdosis,urinary albumin,blood and urine MCP-1,8-iso-prostaglandin F2a（8-iso-PGF2α）and tumor necrosis factor-α（TNF-α）,and oservation were performed on the pathological change of the rats renal with light microscope.Result： Compared with those in the control group,the body weight and kidney weight index were decreased in the Ge Pi Decoction treatment（P 0.05）.Microdosis urin-albumin,MCP-1,8-iso-PGF2α and TNF were decreased significantly（P 0.01）.There was no significant difference between Ge Pi Decoction treatment group and benazepril treatment group（P 0.05）.In addition,there were positive correlation between 8-iso-PGF2α,MCP-1,TNF-α and UAER in each group.Ge Pi Decoction can relieve kidney hypertrophy,mesangial matrix hyperplasia and sclerosis.Conclusion： There are autoimmunity inflammation and oxidative stress in diabetic nephropathy.Ge Pi Decoction can inhibit various inflammatory factors in diabetic nephropathy.

关 键 词：葛脾煎剂 单核细胞趋化蛋白-1 8-异前列腺素F2Α 糖尿病肾病 

分 类 号：R285.5[医药卫生—中药学]