妊娠高血压综合征患者外周血CD62P、CD63检测的临床意义  

Clinical Significance of Measuring Serum CD62P and CD63 in Patients with Pregnancy Induced HypertensionSydrome

作　　者：刘莉莉[1] 晁鹏丽[1] 张忠英[1] 

机构地区：[1]厦门大学附属中山医院检验科,福建厦门361004

出　　处：《国际检验医学杂志》2010年第3期209-211,共3页International Journal of Laboratory Medicine

基　　金：2000年厦门市卫生局重点科研计划项目

摘　　要：目的探讨妊娠高血压综合征(妊高征)患者、正常妊娠早期、中期、晚期妇女血小板活化状态、血小板指标、凝血功能指标的变化,探讨其对妊高征患者血栓前状态的临床预测及诊治价值。方法随机选取22例妊高征患者(妊高征组)、71例正常妊娠妇女(妊娠早期组24例、妊娠中期组23例、妊娠晚期组24例)和22例正常体检妇女(健康对照组)作为研究对象,运用流式细胞仪、血细胞分析仪、血凝仪观察各组血小板上CD62P、CD63的表达及血小板(PLT)、血小板压积(PCT)、血小板体积(MPV)、血小板宽度(PDW)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fbg)等含量。结果妊高征组CD62P含量明显上调,与妊娠早期组、妊娠中期组和妊娠晚期组比较,差异具有统计学意义(分别P<0.01、P<0.01、P<0.05);妊高征组CD63表达量也高于妊娠早期组、妊娠中期组及妊娠晚期组,但差异无统计学意义(P>0.05)。妊娠晚期组CD62P表达量明显高于妊娠早期组及妊娠中期组,差异具有统计学意义(P<0.01)。妊娠晚期组的PLT含量显著高于妊娠早期和妊娠中期组的孕妇(分别P<0.01、P<0.05)。妊高征组PLT含量减少,与妊娠早期组、妊娠中期组及妊娠晚期组相比差异无统计学意义(P>0.05)。PLT与CD62P呈负相关(r=-0.256,P<0.05);PLT与CD63呈负相关(r=-0.336,P<0.01)。CD62P与CD63呈正相关(r=0.303,P<0.01)。CD62P、CD63、PLT、MPV、PCT、PDW、PT、Fbg指标在妊娠晚期组、妊高征组和健康对照组之间差异有统计学意义(P<0.01)。妊娠晚期组MPV、PDW、PT、Fbg与健康对照组相比明显上调,差异具有统计学意义(P<0.01);TT与健康对照组相比明显下调,差异具有统计学意义(P<0.01)。妊高征组CD62P、CD63、PDW、Fbg与健康对照组相比明显上调,差异具有统计学意义(P<0.01);PLT、PCT与健康对照组相比明显下调,差异具有统计学意义(P<0.01)。妊高征组CD6Objective To study the predictive and diagnostic value for pre thrombus status according to the changes of platelet activation, platelet count and parameters, coagulation function parameters in patients with pregnancy induced hypertension （PIH） ,early-pregnant women,middle-pregnant women and late-pregnant women. Methods Twenty-two patients with PIH were selected as research group randomly, The control group included Seventy-one, normal pregnancy women and twenty-two normal non-pregnant women. The expression of CD62P and CD63 in peripheral blood were measured by flow cytometry, the quantities of the blood platelet were determined by Cell-DNY 3700, the antithrombosis and thrombolysis system were measured by thrombosis auto-analyze. Results CD62P in PIH was significant higher than that of early and middle and latepregnancy women （P〈0.01 ,P〈 0.01 ,P〈0.05）. The expression of CD63 in PIH was higher than others normal pregnancy women without significant deference（P 〉0.05）. The CD62P and PLT in late-pregnant women were higher than that of early and middle, pregnant women significantly . The Platelet count in PIH declined, without significant difference comparing with ohters pregnancy women（P〉0. 05）. Platelet count was negative correlated withCD62P（r=-0. 256,P〈0.05） and CD63（r=- 0. 336,P〈0.01） remarkably. The level of CD62P was positive correlated remarkably with CD63（r=0. 303,P〈0.01）. CD62P,CD63,PLT,MPV,PCT,PDW,PT,Fbg existed significant difference among late-pregnant women, PIH and normal controls （P〈0.01 ）. MPV, PDW, PLT, Fbg were increased obvi ously in late-pregnant women （P 〈 0.01 ） comparing with control, but TT was declined significantly （ P 〈 0.01 ）, CD62P, CD63, PDW,Fbg were increased obviously , but PLT and PCT were dropped in PIH women（P〈0.01）comparing with normal control, as to CD62P, it was increased obviously（P〈0.01）, but MPV,PCT,PT,Fhg were dropped in PIH women（P〈0.01）comparing with late-pregnant women. Combined with CD62P and

关 键 词：妊娠并发症 高血压 P选择素 血小板 血液凝固 流式细胞术 

分 类 号：R446.61[医药卫生—诊断学] R714.246[医药卫生—临床医学]