碱性成纤维细胞生长因子缓释支架联合骨髓间质干细胞治疗猪心肌梗死的心肌SPECT显像  

Effect of intramyocardial controlled releasing base fibroblast growth factor stent combined with bone marrow-derived mesenchymal stem cells transplantation on cardiac repair in porcine acute myocardial infarction models

作　　者：赵晓斌[1] 史蓉芳[1] 唐坤[1] 赵健[2] 王颖[2] 刘晓程[2] 

机构地区：[1]天津泰达国际心血管病医院核医学科,天津300457 [2]天津泰达国际心血管病医院心外科,天津300457

出　　处：《中国医学影像技术》2010年第3期410-413,共4页Chinese Journal of Medical Imaging Technology

基　　金：天津市科技发展计划项目(05YFGZSF2900)

摘　　要：目的评价心肌SPECT显像对碱性成纤维细胞生长因子(b-FGF)缓释支架联合骨髓间质干细胞(BM-MSCs)移植治疗中国实验用小型猪急性心肌梗死的价值。方法中国实验用小型猪18头,按完全随机法分为3组:机械打孔+空白支架(对照组)、机械打孔+b-FGF支架(单纯治疗组)、机械打孔+b-FGF支架+BM-MSCs(联合治疗组)。所有猪左前降支均被结扎制作心肌梗死模型。3组均在梗死区及周边区机械打孔并分别埋入空白支架、b-FGF支架、b-FGF支架+BM-MSCs;术后行心肌SPECT显像检测心肌血流的改变,同时还进行超声心动图、免疫组化检查。结果术后6周,治疗组梗死心肌质量差值、短轴缩短率、新生血管密度均高于对照组(P<0.05),且联合治疗组高于单纯治疗组(P<0.05)。结论b-FGF缓释支架联合BM-MSCs能够改善心肌梗死区域血流、促进血管生长、提高心功能;心肌SPECT显像是评估碱性成纤维细胞生长因子缓释支架联合骨髓间质干细胞治疗急性心肌梗死效果有价值的方法。Objective To evaluate the therapeutic effect of intramyocardial controlled releasing base fibroblast growth factor（b-FGF） stent combined with transplanting bone marrow-derived mesenchymal stem cells（BM-MSCs） in porcine acute myocardial infarction（AMI） models.Methods Eighteen porcines were divided into three groups（each n =6）： transmyocardial revascularization（TMR）＋naked stent（control group）,TMR＋b-FGF stent（b-FGF group） and TMR＋b-FGF stent＋BM-MSCs（b-FGF＋BM-MSCs group）.Acute myocardial infarction models were induced by ligating the left anterior descending artery in 18 porcine.In the control and b-FGF group,naked stents,b-FGF stents and b-FGF stent＋BM-MSCs were implanted into TMR channels in the infarct and border zone.99mTc-methoxyisobutylisonitrile（MIBI） myocardial perfusion imaging were performed to evaluate changes in myocardial blood flow,and echocardiography and immunohistochemical studies were also performed.Results After 6 weeks,the quality of myocardial infarction,fractional shortening,neovascular density in b-FGF and b-FGF stent＋BM-MSCs group were higher than those of control gruop（P 0.05）,and in b-FGF stent＋BM-MSCs group was higher than those in b-FGF group（P 0.05）.Conclusion BM-MSCs combined with controlled releasing intramyocardial b-FGF stent can improve the flow of myocardial infarction area,promote angiogenesis and heart function,and the therapeutic effect which can be evaluated with myocardial SPECT imaging.

关 键 词：骨髓间质干细胞移植 碱性成纤维细胞生长因子 体层摄影术 发射型计算机 单光子 猪 

分 类 号：R542.2[医药卫生—心血管疾病] R-332[医药卫生—内科学]