氧化钠胁迫下苯丙烯酸对黄瓜根际细菌DNA分子水平多态性的影响  

Effects of cinnamic acid on bacterial DNA polymorphorism in rhizosphere soil of cucumber under NaCl stress

作　　者：刘静[1] 吴凤芝[1] 杨阳[1] 刘守伟[1] 潘凯[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《应用生态学报》2010年第3期661-667,共7页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目(2009CB119004-05);国家自然科学基金项目(30771252)资助

摘　　要：采用PCR-DGGE技术,研究了NaCl(0、292.5、585mg·kg-1土)胁迫下,不同浓度苯丙烯酸(0、25、50、100、200mg·kg-1土)对黄瓜根际细菌DNA分子水平多态性的影响.结果表明:在黄瓜幼苗的不同时期,低浓度苯丙烯酸(50mg·kg-1土)处理使DGGE图谱中的条带数和条带灰度与对照(CK,0mg·kg-1土)相近,多样性指数、均匀度指数和丰富度指数最高;高浓度苯丙烯酸(100、200mg·kg-1土)处理使土壤DGGE图谱中的条带数减少,条带灰度变暗,多样性指数、均匀度指数和丰富度指数较低.表明NaCl胁迫下,低浓度苯丙烯酸缓解而高浓度苯丙烯酸加重了盐分对土壤微生物的胁迫.对目的条带的克隆测序结果表明,受影响的主要细菌类群多数为不可培养细菌及α-、γ-和β-变形菌门,有少部分属于厚壁菌门、酸杆菌门和放线菌门.By using PCR-DGGE technique, this paper studied the effects of different concentration （0, 25, 50, 100, and 200 mg·kg^-1 soil） cinnamic acid on the bacterial DNA polymorphorism in rhizosphere soil of cucumber seedlings under the stress of 292.5 and 585 mg NaCl·kg^-1 soil. At all growth stages of cucumber seedlings, treatment 50 mg·kg^-1 of cinnamic acid had the similar band numbers and band gray scales in DGGE profiles to treatment 0 mg·kg^-1 of cinnamic acid, but the diversity index, richness index, and evenness index were the highest; while in treatments 100 and 200 mg·kg^-1 soil of cinnamic acid, the band numbers and band gray scales decreased, and the diversity index, richness index, and evenness index were lower. Our results demonstrated that low concentration cinnamic acid relieved the salt stress on soil microbes, while high concentration cinnamic acid aggravated the stress. The cloning and sequencing results showed that the main bacterial groups affected by salt stress were uncultured bacterial species, α-Proteobacteria, β-proteobacteria, and γ-proteobacteria, and a few were Firmicutes, Acidobacteria, and Actinobacteria.

关 键 词：盐分胁迫 苯丙烯酸 PCR-DGGE 土壤细菌 

分 类 号：S642.2[农业科学—蔬菜学]