水稻促分裂原活化蛋白激酶基因OsMPK14的克隆及表达分析  

Cloning and Expression Analysis of a Mitogen-Activated Protein Kinase Gene OsMPK14 from Rice

作　　者：梁卫红[1] 毕佳佳[1] 彭威风[1] 张帆[1] 石宏浩[1] 李莉[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《中国水稻科学》2010年第2期125-130,共6页Chinese Journal of Rice Science

基　　金：教育部科学技术研究重点项目(209076);河南省科技攻关计划资助项目(092102110092);河南省基础与前沿技术研究计划资助项目(092300410099);河南省创新型科技人才队伍建设工程资助项目;河南省教育厅自然科学研究计划资助项目(2010A180012)

摘　　要：促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)是生物体内信号转导的重要组分,受多种生物及非生物胁迫的刺激活化。利用RT-PCR方法克隆了水稻促分裂原活化蛋白激酶基因OsMPK14的cDNA序列(GenBank登录号为GQ265780)。该序列全长1660bp,包含1个1629bp的开放阅读框,编码蛋白由542个氨基酸组成,包含典型的蛋白激酶结构域及磷酸化位点TDY基序。序列比对和分析显示,OsMPK14基因位于水稻第5染色体上,其编码区由9个外显子和8个内含子组成。采用半定量RT-PCR技术,检测了光照、低温、高盐、干旱和脱落酸对该基因在水稻地上部分和根中表达的影响。结果显示高盐、低温、脱落酸都能上调其表达,而干旱对其表达具有微弱的抑制效应,光照可以降低该基因在水稻地上部分的表达,提高在根中的表达。基因可能在水稻非生物胁迫的应答中具有重要作用,其表达受多种因素调控。Mitogen activated-protein kinases （MAPKs） are important components in signal transduction pathways responding to various biotic and abiotic stresses.The cDNA sequence of the MAPK gene OsMPK14 （GenBank Accession No.GQ265780） from rice （Oryza sativa L.） was cloned by RT-PCR.The full-length cDNA of OsMPK14 is 1660 bp in size,containing an open reading frame of 1629 bp,which encodes a 542-amino-acid polypeptide and has a typical protein kinase domain and a phosphorylation activation motif TDY.The sequence alignment and analysis revealed that OsMPK14 is located on chromosome 5,and composed of 9 exons and 8 introns in the coding region.Semi-quantitative RT-PCR was performed to examine its expression patterns in rice shoots and roots under darkness,drought,high salinity,low temperature and abscisic acid treatments.The OsMPK14 mRNA was induced by abscisic acid,low temperature and high salinity,but weakly inhibited by drought.Light could up-regulate its expression in roots,but down-regulate in shoots.The results indicate that OsMPK14 could be implicated in diverse rice stimuli-responsive signaling cascades,and its expression might be regulated by multiple factors.

关 键 词：水稻 促分裂原活化蛋白激酶基因 基因克隆 逆境胁迫 表达分析 

分 类 号：S511[农业科学—作物学]