慢性HBV感染者外周血Vα24 NKT细胞数量变化及其与肝脏损害程度的关系  

Correlation of peripheral blood Vα24 NK T cell count with the severity of hepatic injury in patients with chronic hepatitis B virus infection

作　　者：黄晓刚[1] 苏毅[1] 林世德[1] 龙骏[1] 陈应华[1] 刘士荣[1] 

机构地区：[1]遵义医学院第一附属医院感染科,贵州省遵义市563003

出　　处：《世界华人消化杂志》2010年第6期601-605,共5页World Chinese Journal of Digestology

基　　金：贵州省优秀教育科技人才省长基金资助项目;No.黔省专合字2009[50]~~

摘　　要：目的:探讨慢性HBV感染者外周血Vα24自然杀伤T细胞(NKT)数量变化及其与肝脏损伤程度的关系.方法:观察对象为慢性乙型肝炎(CHB)患者26例、乙型肝炎后肝硬化患者(LC)9例、慢加急性肝衰竭患者(ACLF)8例和7名正常对照组(NC),入院后次日清晨取静脉全血2mL,裂解红细胞后,采用流式细胞仪检测外周血Vα24 NKT细胞数量占外周血T淋巴细胞的比例,同时检测肝功能、凝血功能判断肝脏损害程度.结果:CHB,ACLF组患者外周血Vα24 NKT细胞数量分别为0.8012%±0.2979%、0.4638%±0.2244%,明显低于正常对照组(1.1114%±0.3546%,P<0.05);CHB中度及重度患者NKT数(0.7344%±0.2441%,0.6925%±0.3612%)明显低于正常对照组.NKT数与凝血酶原活动度(PTA)呈显著正相关,与血清总胆红素(TB)呈显著负相关.结论:慢性HBV感染者外周血Vα24 NKT细胞数明显降低,并随肝损害程度加重而减少,提示Vα24NKT细胞可能参与CHB患者肝细胞损伤.AIM： To investigate the changes in the number of Vα24 natural killer （NK） T cells in peripheral blood and its possible correlation with the severity of hepatic injury in patients with chronic hepatitis B virus （HBV） infection. METHODS： Twenty-six patients with chronic hepatitis B （CHB）, 9 patients with liver cirrhosis （LC）, 8 patients with acute-on-chronic liver failure （ACLF）, and 7 normal controls （NCs） were included in the study. The percentage of Vα24 NK T cells in total peripheral blood T lympho-cytes was determined by flow cytometry.RESULTS： The percentages of Vα24 NKT cells in patients with CHB and ACLF were signifi-cantly lower than that in NCs （0.8012% ± 0.2979% and 0.4638% ± 0.2244% vs 1.1114% ± 0.3546%, respectively, both P 0.05）. The percentage of Vα24 NK T cells had a significant positive correlation with prothrombin time （PT） and a significant negative correlation with total bilirubin （TB）, but had no significant correlations with serum alanine aminotransferase （ALT） and HBV DNA level. CONCLUSION： The percentage of Vα24 NK T cells in peripheral blood decreases with the aggravation of hepatic injury in patients with chronic HBV infection, implying that Vα24 NKT cells play an important role in the pathogenesis of chronic hepatitis B.

关 键 词：Vα24自然杀伤T细胞 乙型肝炎 流式细胞术 

分 类 号：R512.62[医药卫生—内科学] R575[医药卫生—临床医学]