体外快速减肥模型的建立及初步评价  

Evaluation on a fast weight reduction model in vitro

作　　者：李松涛[1] 李颖[1] 闻颖[1] 孙长颢[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,哈尔滨150086

出　　处：《卫生研究》2010年第2期159-161,共3页Journal of Hygiene Research

基　　金：国家"十一五"科技支撑计划项目(No.2006BAD27B01;No.2006BAD27B05)

摘　　要：目的建立一种快速、高效的减肥药物体外筛选模型,并对该模型进行初步评价。方法用油酸诱导SD大鼠原代成熟脂肪细胞建立肥胖细胞模型,选用阳性药物异丙肾上腺素和已明确有减肥作用的功能因子染料木素、咖啡因及阴性对照物姜黄素对该肥胖模型进行评价。结果试验所选用的阳性受试物均能显著刺激脂肪细胞发生脂解,而阴性受试物无脂解作用。结论该模型可以用于减肥药物的体外快速筛选。Objective To establish a fast and effective model in vitro for screening weight - reducing drugs and taking preliminary evaluation of the model. Methods Mature adipocytes of SD rat induced by oleic acid were used to establish a obesity model in vitro. Isoprel,genistein,caffeine were selected as positive agents and curcumine as negative agent to evaluate the obesity model. Results Lipolysis of adipocytes was stimulated significantly by isoprel, genistein and caffeine rather than curcumine. Conclusion This model could be used efficiently for screening weight- losing drugs.

关 键 词：减肥模型 油酸 脂肪细胞 脂解 体外 

分 类 号：R589.2[医药卫生—内分泌]